buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
EGTA (2X) Buffer, Enzo Life Sciences
Supplier: Enzo Life Sciences
EGTA buffer, 2X
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Proteasome assay buffer
Supplier: Enzo Life Sciences
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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MMP-1 colorimetric assay buffer
Supplier: Enzo Life Sciences
MMP-1 colorimetric assay buffer
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Blocking buffers
Supplier: Rockland Immunochemicals
A blocking buffer specifically designed for western blotting using fluorochrome conjugated antibodies. Blocking buffer was prepared using ultra pure reagents dissolved in pharmaceutical grade water (WFI) and consists of a proprietary protein formulation in tris buffered saline at pH 7,6 with thimerisol added as an antimicrobial agent.
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Phosphate Buffered Saline
Supplier: THERMO FISHER DIAGNOSTICS
Cell culture buffer, Phosphate buffered saline tablets
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Dry buffer packs, JAW™
Supplier: G-Biosciences
Premixed buffer powders that simply require adding water to generate a ready-to-use buffer.
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IHC Wash Buffer, Enzo Life Sciences
Supplier: Enzo Life Sciences
The IHC wash buffer is a for washing and removing excess reagents during immunostaining.
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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Wash buffer concentrate
Supplier: Enzo Life Sciences
Liquid. TRIS buffered saline containing detergents.
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Gram Negative lysis buffer
Supplier: G-Biosciences
Gram-negative lysis buffer is an extraction buffer for soluble proteins from Gram-negative bacteria. It is a proprietary improvement on the lysozyme based lysis in combination with various salts and agents, which allows extraction of soluble proteins. This buffer has been tested for use with several widely used bacteria including E.coli strains.
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TE Buffer (1X) pH 8,0 for Molecular Biology
Supplier: Apollo Scientific
Cloning Buffer, TE buffer (1X)
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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VWR® UltraPure, Buffer, concentrate (phosphate buffered saline)
Supplier: VWR Chemicals
Washing buffer, PBS buffer solution 10X concentrate (phosphate buffered saline)
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VWR® Separating Gel Buffer, 4X
Supplier: VWR Chemicals
4X concentrated buffer solution (1,5 M Tris, pH 8,8 and 0,4% SDS). Used for preparation of standard acrylamide:bis-acrylamide gels as well as preparation of gels using PAGE-PLUS™ acrylamide solution.
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