366 Results for: "VARLP080520VARI00001"

Supplier: SIGMA ALDRICH MICROSCOPY

The combination of azure A and silver staining is used for the quantification of glycosaminoglycans in agarose gels. It is also used for the staining of chromosomes in the nucleus. Azure A is a main component of the azure B/polychromed methylene blue-eosin stains family, also referred to as the Romanowsky stain. Azure A is a basic cationic dye.

Supplier: SIGMA ALDRICH MICROSCOPY

Fast green FCF, Sigma-Aldrich®

Supplier: Merck

The Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative.

Supplier: SIGMA ALDRICH MICROSCOPY

Mixture of crystal violet [C.I.No. 42555] and methyl violet [C.I.No. 42535]. Dye employed for preparing Gram stain.

Supplier: SIGMA ALDRICH MICROSCOPY

Sunset yellow FCF is a food dye, which is is substantially used in various food preparations. It is a orange-red coloured crystal, that is soluble in water and ethanol. It is used in treating bone metabolic diseases, dermatological disorders, respiratory illness etc. It can alter the functional responses of splenocytes at non-cytotoxic dose. It promotes immune suppression. Relative expression of surface receptors in T and B cells can be altered by sunset yellow FCF.

Supplier: SIGMA ALDRICH MICROSCOPY

Silver nitrate along with iodine are among several inorganic substances used to induce colour in microscopical preparations. Silver nitrate as a biological stain exploits the strong colors of colloidal metallic silver. Silver nitrate is an important component of the silver staining that is used for the detection of proteins separated by polyacrylamide gel electrophoresis. Silver nitrate is a component of the silver stain kit. It is required for image development in diamine as well as non-diamine silver staining.

Supplier: SIGMA ALDRICH MICROSCOPY

3-(2-Pyridyl)-5,6-diphenyl-1,2,4-triazine-4′,4′′-disulfonic acid sodium salt is a chelating agent for ferrous iron. It is also known as Ferrozine.

Supplier: SIGMA ALDRICH MICROSCOPY

Chrysin has been used to study the effect of bioflavonoids on the inhibition of ATP synthase in Escherichia coli. It has been used to study the effect of Chrysin on eIF4E (eukaryotic translation initiation factor 4E) expression in AGS cell (human gastric carcinoma cell) line. It has been used to study the effect of chrysin on human ovarian cancer cells.

Supplier: SIGMA ALDRICH MICROSCOPY

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. It is a metachromatic dye suitable for a wide variety of histological staining procedures. A metachromatic dye suitable for a wide variety of histological staining procedures. Toluidine Blue O has been used to stain a variety of specimens like mesenchymal cells, taste buds, osteoblasts, matrix, and more.

Supplier: SIGMA ALDRICH MICROSCOPY

Acriflavine also interacts with HIF-1α (hypoxia-inducible factor 1 α) and HIF-2α, and thereby suppresses dimer formation of HIF-1 and transcriptional function. It also has negative effect on tumor growth and vascularization. Acriflavine also exhibits trypanocidal, antibacterial and antiviral activities.

Supplier: SIGMA ALDRICH MICROSCOPY

Light green SF yellowish, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl Blue is a triaminotriphenylmethane dye, widely used in paper, textile and leather production.

Supplier: SIGMA ALDRICH MICROSCOPY

Acridine Orange base (3,6-Bis(dimethylamino)acridine) is a cell-permeable fluorescent dye that binds to nucleic acids. It emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. It is also known to be a positive solvatochromic dye. Acridine orange has also been used as a lysosomal dye. It is widely used to detect the presence of homeopathic potencies.

Supplier: SIGMA ALDRICH MICROSCOPY

Crystal violet has been used to determine cell viability. It has been used in the staining of cells for the quantification of cellular migration.

Supplier: SIGMA ALDRICH MICROSCOPY

Phenol Red sodium salt is widely used as a pH indicator. At a pH of 6,4 and below, phenol red solution will have a yellow colour. At a pH of 8,2 and above, it will have a red colour. It is commonly used in culture media to detect neutral to acidic pH changes.

Supplier: SIGMA ALDRICH MICROSCOPY

Sudan Red 7B has been used to stain the fat bodies of Ostrinia nubilalis (Hübner) larvae. Sudan red 7B, also known as fat red, is a non-fluorescent stain.[2] It is effectively used to stain lipids and is used to indicate the presence of suberin in cell walls.[2][3][4] Sudan red 7B acts as a marker for formosan subterranean termite, Coptotermes fornosanus Shiraki.

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein isothiocyanate (FITC) is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been used for the staining of conidia.
Reagent for the FITC labeling of proteins; Microsequencing of proteins and peptides (HPLC).

Supplier: SIGMA ALDRICH MICROSCOPY

Fluorescein isothiocyanate (FITC) is yellow-orange in color with an absorption maximum at 495nm. Upon excitation, it emits a yellow-green color with an emission maximum at 525 nm. It is widely used to attach a fluorescent label to proteins via the amine group. The isothiocyanate group reacts with amino terminal and primary amines in proteins. It has been used for the labeling of proteins including antibodies and lectins. Fluorescein isothiocyanate isomer I has been proposed as a contact sensitiser.

Supplier: SIGMA ALDRICH MICROSCOPY

Nitrotetrazolium blue (NBT), in combination with BCIP (5-bromo-4-chloro-3-indolyl-phosphate), is required for the detection of labeled probes in western blots. It is part of the colorimetric detection assay. NBT and BCIP dyes detect alkaline phosphatase activity, thereby generating an insoluble purple dye (diformazan). The dye is visible at areas where the labeled probe has hybridised to its target.

Supplier: SIGMA ALDRICH MICROSCOPY

Phenol Red Sodium Salt (PRSS) is a water-soluble anionic fluorescent dye, that possesses an extra Na ion attached with the SO3 group compared to phenol red. It is intended for use in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6,4 or below and a red color at a pH of 8,2 and above.

Supplier: SIGMA ALDRICH MICROSCOPY

Safranin O, also known as basic red 2, is a basic, cationic, and metachromatic azine dye used as a biological stain. The characteristic azine chromophore contains the phenazine, a planar, tricyclic dibenzopyrazine, which consists of two non-planar benzene rings linked by two nitrogen atoms. A delocalised positive charge, an alternation of aromatic and quinonoid structures, and diazotisable amine groups constitute the chromophoric system. c-Methylation makes this dye of moderate overall size. The trimethyl cationic form of safranine is lipophilic, whereas the dimethyl forms are weakly hydrophilic. The dye is commercially available as a chloride.

Supplier: SIGMA ALDRICH MICROSCOPY

4-Amino-3-hydroxy-1-naphthalenesulfonic acid is a derivative of aniline consisting of a trifunctional monomer (bearing three functional groups –NH₂, –OH and –SO₃H). It also contains two fused benzene rings.

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin Y is a xanthene dye and is used for the differential staining of connective tissue and cytoplasm. In histopathology, it is applied as a counterstain after hematoxylin and before methylene blue. It is also used as a background stain, thereby giving contrast to the nuclear stains.

Supplier: SIGMA ALDRICH MICROSCOPY

Nuclear Fast Red is suitable for the determination of calcium. It is suitable for use in prussian blue staining in neural progenitor cells. It may be employed as highly sensitive 'off/on' fluorescent probe for the determination of guanine. Nuclear fast red (2-anthracenesulfonic acid, 4-amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-, monosodium salt) may be used as selective complexing agent for the simultaneous determination of copper and bismuth by adsorptive stripping voltammetry.

Supplier: SIGMA ALDRICH MICROSCOPY

Giemsa stain is a versatile differential stain that is widely used in hematology for blood and bone marrow specimens, clinical cytology specimens, bacteriology, histological biopsies, and tumor samples. Giemsa's staining solution is composed of methylene blue, azure, and eosin. It is a major histological stain due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. The stain is frequently used in combination with other dye solutions: May-Grünwald’s solution for Pappenheim (MGG) and Wright-Giemsa.

Supplier: SIGMA ALDRICH MICROSCOPY

Basic fuchsin is a triaminotriphenylmethane dye that is widely used to stain muscle, mitochondria, and collagen. It is generally a mixture of pararosaniline (C.I. 42500), rosaniline (C.I. 42510), magenta II and new fuchsine (CI 42520).

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

Supplier: SIGMA ALDRICH MICROSCOPY

Eosin Y, also known as Eosin yellowish is a widely used xanthene dye. It is a counterstain that is used to distinguish between various connective tissue fibers and matrices, and the cytoplasm of different types of cells. Eosin in combination with hematoxylin is useful to demonstrate the general histological architecture of a tissue.

Supplier: SIGMA ALDRICH MICROSCOPY

Orcinol is a small-molecule natural phenol involved in the production of a dye called orcein used for the staining of cells and chromosomes. Orcinol monohydrate is usually used to prepare a colorimetric reagent known as Bial's reagent for the determination of RNA, DNA, and hexoses.