"ImmunoReagents"

Supplier: ImmunoReagents

The ImmunoReagents' DyLight 405 has a vibrant blue fluorescence intensity that replaces Alexa Fluor™ 405, Cascade™ Blue and AMCA in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight® 405 (Ex = 400 nm; Em = 420 nm)

Supplier: ImmunoReagents

The ImmunoReagents' DyLight® 594 conjugates generate red fluorescence and shows wide excitation wavelength separation from green fluorophores for multi-Colour applications with reduced bleedthrough. The physical characteristics of DyLight® 594 are comparable to other 594 dyes. DyLight® 594 is available for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms. Excitation/Emission = 593 nm/618 nm Emission Colour = Red (Similar Dyes: Alexa Fluor 594, Texas Red).

Supplier: ImmunoReagents

The ImmunoReagents' DyLight® 800 conjugates uses a near-IR fluorphore that is invisible to the naked eye but increases the staining options when using infrared imaging systems. DyLight® 800 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor 790 and IRDye 800, and has high solubility for maximal incorporation of the dye into the biomolecule of interest. Excitation/Emission = 777 nm / 794 nm Emission Color = Near IR (Similar Dyes: Alexa Fluor 790, IRDye 800)

Supplier: ImmunoReagents

ImmunoReagents’ Biotinylated antibody conjugates are widely used due to their ability to enhance antibody signal in most immunoassays. Biotinylated secondary antibodies are used in most immunoassay formats (i.e., ELISA, Immunohistochemistry (IHC), and Western blotting). While Biotinylated antibodies by themselves do not produce a signal that can be visualized, biotin has a high affinity and specificity to Streptavidin/Avidin. Using ImmunoReagents’ streptavidin conjugates with biotinylated antibodies results in signal amplification.

Supplier: ImmunoReagents

The ImmunoReagents' DyLight® 594 conjugates generate red fluorescence and shows wide excitation wavelength separation from green fluorophores for multi-color applications with reduced bleedthrough. The physical characteristics of DyLight® 594 are comparable to other 594 dyes. DyLight® 594 is available for use in fluorescence microscopy, flow cytometry, Western blotting, ELISA, high-content screening and other array platforms. Excitation/Emission = 593 nm / 618 nm Emission Color = Red (Similar Dyes: Alexa Fluor 594, Texas Red)

Supplier: ImmunoReagents

TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in color but emits a red-orange color upon Emission at 580nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second color detection reagent in in situ hybridization applications. (Excitation/Emission = 555 nm / 580 nm Emission Color = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550).

Supplier: ImmunoReagents

Anti-IgG Mouse Polyclonal Antibody

Supplier: ImmunoReagents

ImmunoReagents FITC Conjugates are used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. Fluorescein is typically excited by the 488 nm line of an argon laser, and emission is collected at 518 nm. FITC antibody conjugates are yellow-orange in color but emit a yellow-green color upon Em 518 nm. FITC antibody conjugates are sensitive to light. The typical FITC to antibody ratio (F/P) is >4.0 Excitation/Emission = 494 nm / 518 nm Emission Color = Green (Similar Dyes: Alexa Fluor 488, Cy2, DyLight ® 488).

Supplier: ImmunoReagents

TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in Colour but emits a red-orange Colour upon Emission at 580 nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second Colour detection reagent in in situ hybridization applications. (Excitation/Emission = 555 nm/580 nm Emission Colour = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550).

Supplier: ImmunoReagents

Anti-IgG Goat Polyclonal Antibody (DyLight® 633)

Supplier: ImmunoReagents

ImmunoReagents’ Horseradish Peroxidase (HRP) antibody conjugates are the most popular conjugates due to their high level of sensitivity, stability, and low costs. HRP conjugates are an ideal choice for use in ELISA, Immunohistochemistry (IHC), and Western blotting experiments.

Supplier: ImmunoReagents

TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in colour but emits a red-orange colour upon Emission at 580 nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second colour detection reagent in in situ hybridisation applications. (Excitation/Emission = 555 nm/580 nm Emission Colour = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550)

Supplier: ImmunoReagents

ImmunoReagents’ Horseradish Peroxidase (HRP) antibody conjugates are the most popular conjugates due to their high level of sensitivity, stability, and low costs. HRP conjugates are an ideal choice for use in ELISA, Immunohistochemistry (IHC), and Western blotting experiments.

Supplier: ImmunoReagents

ImmunoReagents’ Horseradish Peroxidase (HRP) antibody conjugates are the most popular conjugates due to their high level of sensitivity, stability, and low costs. HRP conjugates are an ideal choice for use in ELISA, Immunohistochemistry (IHC), and Western blotting experiments.

Supplier: ImmunoReagents

The ImmunoReagents' DyLight 680 produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5.5™ dye and Alexa Fluor™ 680, and is ideal for multi-color applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight® 680 (Ex = 682 nm; Em = 715 nm)

Supplier: ImmunoReagents

ImmunoReagents’ Horseradish Peroxidase (HRP) antibody conjugates are the most popular conjugates due to their high level of sensitivity, stability, and low costs. HRP conjugates are an ideal choice for use in ELISA, Immunohistochemistry (IHC), and Western blotting experiments.