2126 Results for: "Cytiva"
Blotting membranes, Amersham™ Hybond™ 0,45 PVDF
Supplier: Cytiva
Amersham™ Hybond™ P 0,45 PVDF is a 0,45 μm pore size hydrophobic membrane, with high physical strength, highly suitable for stripping and reprobing. For use in chemiluminescent and fluorescent detection methods for proteins of >Mr 20000.
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Sera-Mag carboxylate-modified magnetic beads and SpeedBeads
Supplier: Cytiva
Sera-Mag Carboxylate-Modified Magnetic Beads & SpeedBeads have free carboxyl groups on the surface for convenient covalent coupling of target molecules (proteins, peptides, and amine-modified ligands) via primary amines (NH2).
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illustra™ ProbeQuant™ G-50 micro columns
Supplier: Cytiva
ProbeQuant™ G-50 micro columns use spin column chromatography to provide a fast and reliable method for purifying labelled probes from unincorporated labelled nucleotides. In addition, a portion of the eluted DNA can be counted in a scintillation counter and compared to an unpurified sample to give an approximation of percent incorporation. The columns are designed for use in a microcentrifuge, come prepacked with Sephadex™ G-50 DNA grade and are pre-equilibrated in STE, which means that they are ready to use. They can accommodate samples ranging in volume from 25 μl to 50 μl.
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Affinity chromatography columns, HisTrap™ FF
Supplier: Cytiva
HisTrap™ FF columns are designed for simple, one-step purification and scaling up purification of histidine-tagged proteins. The columns are pre-packed with Ni Sepharose™ 6 Fast Flow.
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Affinity chromatography columns, HiTrap™ NHS-Activated HP
Supplier: Cytiva
HiTrap NHS-activated HP prepacked chromatography columns allow you to design an affinity column suitable for your purification needs.
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Amersham™ Protran® Western Blotting Membranes, Nitrocellulose
Supplier: Cytiva
Amersham™ Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible. They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pretreatment steps are necessary.
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Protein G Sepharose™ 4 Fast Flow Affinity Chromatography Media
Supplier: Cytiva
Protein G Sepharose 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose 4 Fast Flow.
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Affinity coupling media, NHS-Activated Sepharose™ 4 Fast Flow
Supplier: Cytiva
NHS-activated Sepharose™ 4 Fast Flow is composed of cross-linked 4% agarose beads, NHS activated for coupling amino-containing proteins and peptides in process-scale applications. NHS (N-hydroxysuccinimide) coupling forms a chemically stable amide bond with ligands containing primary amino groups. The resin provides a spacer arm to the coupled ligand and suitable for immobilising smaller protein and peptide ligands.
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Columns, PD MiniTrap™ G-25
Supplier: Cytiva
PD MiniTrap G-25 columns are prepacked, single-use gravity columns for desalting, buffer exchange, and sample cleanup.
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Columns, PD SpinTrap™ G-25
Supplier: Cytiva
PD SpinTrap G-25 is a single-use column for cleanup of large biomolecules.
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ETFE tubing
Supplier: Cytiva
Ethylenetetrafluorethylene tubing (ETFE) is designed for use with AKTA systems.
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HiLoad Superdex columns, 75 PG
Supplier: Cytiva
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component
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Affinity chromatography columns, HiTrap™ TALON® crude
Supplier: Cytiva
HiTrap TALON® crude 1 and 5 ml columns allow for rapid separations and easy scale-up with a minimum of sample preparation and equipment.
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Affinity coupling media, CNBr-Activated Sepharose™ 4B
Supplier: Cytiva
CNBr-activated Sepharose™ 4B is pre-activated media used for coupling antibodies or other large proteins containing -NH₂ groups to the Sepharose™ media, by the cyanogen bromide method, without an intermediate spacer arm.
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illustra™ TempliPhi™100/500 Amplification Kits
Supplier: Cytiva
TempliPhi™ Kits efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30 °C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 minutes of hands-on time to amplify 96 samples from bacterial colonies.
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HiLoad Superdex columns, 200 PG
Supplier: Cytiva
Superdex prep grade (pg) is produced by covalent bonding of dextran to highly cross-linked agarose.The separation properties of these media are determined by the dextran component
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illustra™ MicroSpin™ G-25 columns
Supplier: Cytiva
For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin column chromatography.
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Thrombin
Supplier: Cytiva
Thrombin is a protease used to digest fusion proteins prepared from pGEX vectors containing the recognition sequence for thrombin (pGEX-1lT, pGEX-2T, pGEX-2TK, pGEX-4T-1, pGEX-4T-2, and pGEX-4T-3).
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Anti-IgG Donkey Polyclonal Antibody (HRP (Horseradish Peroxidase))
Supplier: Cytiva
Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.
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Small-scale SEC columns, Superose 6 Increase
Supplier: Cytiva
Superose 6 Increase SEC columns are designed for high-resolution, small-scale preparative purification, and analysis of large globular proteins, protein complexes, and other macromolecules in the molecular weight range 5 to 5000 kD.
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Affinity chromatography columns, HisTrap™ excel
Supplier: Cytiva
HisTrap excel columns are prepacked with Ni Sepharose excel affinity resins for capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants by immobilized metal ion affinity chromatography (IMAC).
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Accessories for chromatography systems
Supplier: Cytiva
Flow restrictor, FR-902
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PCR beads, illustra™ PuReTaq Ready-To-Go™
Supplier: Cytiva
PuReTaq RTG PCR beads and reagents ensures reliable and robust performance in both end point and RT-PCR amplifications and ensures the lowest possible levels of contaminating prokaryotic and eukaryotic nucleic acids.
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Molecular weight markers, Rainbow™
Supplier: Cytiva
Rainbow™ molecular weight markers enable faster and simpler identification of proteins on SDS polyacrylamide gels. These ready to load markers provide sharper, more intense bands on gels and blots, and have improved band spacing for more accurate molecular weight determination. The bright, distinctive colours of the markers allow easier confirmation of transfer to blotting membranes and orientation.
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Film, Amersham™ Hyperfilm™ ECL™
Supplier: Cytiva
Amersham™ Hyperfilm™ ECL™ is constructed from a clear base coated on both sides with a photographic emulsion that is protected by an anti-scratch layer. The anti-scratch coating reduces the risk of visible artefacts (e.g., plastic wrap marks) and the clear colourless base results in easy reading of images without compromising on sensitivity.
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Size exclusion chromatography columns, Superdex™ 75 Increase
Supplier: Cytiva
Superdex 75 Increase 10/300 GL, 5/150 GL, and 3.2/300 are prepacked columns for versatile use in size exclusion chromatography applications.
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Amersham™ Protran® Premium Western Blotting Membranes, Nitrocellulose
Supplier: Cytiva
Amersham™ Protran® Premium nitrocellulose blotting membranes provide excellent sensitivity, resolution and low background in fluorescence Western blotting detection applications.
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Biacore consumables and accessories
Supplier: Cytiva
Biacore maintenance kits are convenient kits for proper instrument maintenance.
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Cation exchange chromatography columns, HiTrap SP HP
Supplier: Cytiva
HiTrap SP HP is a strong cation exchange chromatography column for high-resolution, small-scale protein purification.
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Magnetic rack, MagRack™ 6
Supplier: Cytiva
MagRack 6 is a magnetic rack for small-scale protein purification and sample enrichment with magnetic beads. Each rack consists of anodized aluminium housing (blue), detachable plastic bar (white) containing a neodymium magnet.