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Western blotting blocking reagent, Amersham ECL™ Prime

Western blotting blocking reagent, Amersham ECL™ Prime

Supplier: Cytiva

Amersham ECL Prime Blocking Reagent is blocking Amersham Protran NC and Amersham Hybond PVDF membranes used with Amersham ECL start, Amersham ECL, Amersham, ECL Prime, Amersham ECL Select and Amersham ECL Plex.

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Anti-IgG Donkey Polyclonal Antibody (HRP (Horseradish Peroxidase))

Supplier: Cytiva

Highly species specific HRP-conjugated antibodies optimized for use with Amersham ECL Western Blotting Detection Reagents.

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Affinity chromatography column, HisPrep™ FF

Affinity chromatography column, HisPrep™ FF

Supplier: Cytiva

HisPrep FF 16/10 is a ready-to-use column, prepacked with precharged Ni Sepharose 6 Fast Flow. The column is an excellent choice for preparative, scale-up purification of histidine-tagged recombinant proteins.

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Gel filtration columns, HiPrep™ with Sephacryl® S-200 HR

Gel filtration columns, HiPrep™ with Sephacryl® S-200 HR

Supplier: Cytiva

HiPrep™ 16/60 Sephacryl® S-200 HR and HiPrep™ 26/60 Sephacryl® S-200 HR are pre-packed gel filtration columns for preparative separation of biomolecules of up to 5 and 13 ml sample volume respectively. Sephacryl® S-200 is used for separating mid-size proteins including antibodies and serum proteins.

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illustra™ MicroSpin™ S-400 HR columns

illustra™ MicroSpin™ S-400 HR columns

Supplier: Cytiva

illustra™ MicroSpin™ S-400 HR columns designed for rapid purification of PCR products (>200 bp) from unincorporated primers (<32-mers) and nucleotides using spin-column chromatography.

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illustra™ GFX™ PCR DNA and gel band purification kit

illustra™ GFX™ PCR DNA and gel band purification kit

Supplier: Cytiva

The illustra GFX PCR DNA and Gel Band Purification kits are for the isolation and concentration of DNA fragments from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction enzyme digests.

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Accessories for ÄKTA chromatography systems

Accessories for ÄKTA chromatography systems

Supplier: Cytiva

Inline filter kit (10 ml/min systems)

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Affinity chromatography media, Glutathione Sepharose™ 4B

Affinity chromatography media, Glutathione Sepharose™ 4B

Supplier: Cytiva

Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

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Blotting membranes, Amersham™ Hybond™-N+

Blotting membranes, Amersham™ Hybond™-N+

Supplier: Cytiva

Amersham™ Hybond™-N+ is a positively charged nylon membrane with a binding capacity for nucleic acids up to 600 µg/cm² recommended for use with radioactive or non radioactive chemiluminescence and chemifluorescence detection systems.

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Percoll™ PLUS

Percoll™ PLUS

Supplier: Cytiva

Percoll® PLUS is for preparation of cells, subcellular particles and larger viruses.

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Ficoll® PM400

Ficoll® PM400

Supplier: Cytiva

Ficoll® PM400 is a synthetic neutral, highly branched hydrophilic polymer of sucrose with an average molecular weight of 400000. It is used to form density gradients for separating and isolating eukaryotic cells, organelles and bacterial cells, as a stabilising agent and as a preparation medium for isolating mononuclear cells. It is useful for separating cells that are sensitive to centrifugation. Ficoll™ PM400 can also be used in other applications, such in defined culture media, nucleic acid hybridisation, electrophoresis, and immunological studies.

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Western blotting blocking reagent, Amersham™ ECL™

Western blotting blocking reagent, Amersham™ ECL™

Supplier: Cytiva

Amersham ECL Blocking Agent is for blocking nitrocellulose and PVDF membranes to be used with Amersham ECL, Amersham ECL Prime and Amersham ECL Select Western Blotting Detection Reagents

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Western blotting detection reagent, Amersham™ ECL™ Prime

Western blotting detection reagent, Amersham™ ECL™ Prime

Supplier: Cytiva

Amersham™ ECL™ Prime is a highly sensitive Western blotting detection reagent characterised by stable signal emission, allowing for the possibility of repeated exposures and making it easier to process several blots in the same experimental run. Amersham™ ECL™ Prime delivers a detection system that is sensitive, stable, precisely quantitative across a wide dynamic range of protein levels, and conservative in its consumption of expensive antibody reagents. The high performance of Amersham™ ECL™ Prime is due to the presence of an enhancer in the reagent, increasing enzyme turnover, and thereby significantly increasing both signal intensity and duration. The light signal is further intensified by the addition of a catalyst.

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Affinity chromatography media, Streptavidin Sepharose™ High Performance

Affinity chromatography media, Streptavidin Sepharose™ High Performance

Supplier: Cytiva

Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.

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Anion exchange chromatography columns, HiTrap Q HP

Anion exchange chromatography columns, HiTrap Q HP

Supplier: Cytiva

HiTrap® Q HP are prepacked, ready-to-use High Performance strong anion exchange columns for high-resolution, small-scale protein purification.

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Accessories for empty columns HiScale™

Accessories for empty columns HiScale™

Supplier: Cytiva

Union Valco F/F, PEEK, for HiScale™ 16 and 26

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