"Biotium"
Wheat germ agglutinin (WGA) conjugates
Supplier: Biotium
A wheat germ agglutinin (WGA) lectin labelled with Biotium’s CF® Dyes or HRP. It is a carbohydrate-binding lectin that has high affinity for sialic acid and N-acetylglucosamine moieties of glycoproteins. As a result, WGA conjugates will label yeast bud scars and the cell membrane of gram-positive bacteria and mammalian cells.
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One-Step Blue® protein gel stain
Supplier: Biotium
One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing.
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Anti-KRT19 Mouse Monoclonal Antibody (CF488A) [clone: KRT19/799]
Supplier: Biotium
Recognizes a protein of 40 kDa, identified as cytokeratin-19 (CK19), which is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer.
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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™
Supplier: Biotium
EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.
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Hydroxystilbamidine bis(methanesulphonate) 4% in water fluorescent dye, for histology
Supplier: Biotium
Hydroxystilbamidine (also called Fluoro-Gold™) has been used extensively as a retrograde tracer for neurons and also a histochemical stain. The 4% solution in deionized H2O is a convenient, ready-to-use form..
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Nucleic acid stains, PAGE GelRed™
Supplier: Biotium
Stains specifically designed for DNA in polyacrylamide gels.
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Lipofuscin autofluorescence quencher, TrueBlack™
Supplier: Biotium
TrueBlack™ is a reagent for quenching lipofuscin autofluorescence in tissue sections for immunofluorescence staining. Lipofuscin consists of highly autofluorescent granules of oxidized proteins and lipids that build up in the lysosomes of aging cells. Lipofuscin granules fluoresce brightly in all channels used for fluorescence microscopy and accumulate in a cell and tissue types with age.
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PMA (Propidium monoazide) 20 mM in water DNA/RNA binding dye
Supplier: Biotium
PMA (propidium monoazide) dye is a DNA modifier invented by scientists at Biotium. It is a photo-reactive dye that binds to dsDNA with high affinity. Upon photolysis with visible light, PMA dye covalently attaches itself to dsDNA.
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Cell viability assay kits
Supplier: Biotium
MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.
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Pluronic® F-127 20% in DMSO
Supplier: Biotium
Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as cell-permeable AM esters of Fura-2, Fluo-3, Indo-1, or Rhod-2 calcium indicators to facilitate cell loading.
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Paraformaldehyde 4% in PBS ready-to-use, fixative reagent
Supplier: Biotium
Paraformaldehyde, 4% in PBS is a ready-to-use fixation solution for cells or tissues.
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PMA (Propidium monoazide) DNA/RNA binding dye
Supplier: Biotium
PMA (propidium monoazide) is a high-affinity photoreactive DNA binding dye invented by scientists at Biotium for viability PCR of bacteria and other organisms. The dye is weakly fluorescent by itself but becomes more fluorescent after binding to nucleic acids.
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Fluorometric polymerase activity assay kit, EvaEZ™
Supplier: Biotium
EvaEZ™ Fluorometric Polymerase Activity Assay Kit provides an easy and accurate way to determine the activity of a nucleic acid polymerase without using radioisotopes.
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SYBR® Green I 10000x in DMSO nucleic acid gel stain
Supplier: Biotium
Thiazole green, which is structurally identical to SYBR® green I nucleic acid gel stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.
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PMAxx™ dye 20 mM in water
Supplier: Biotium
PMAxx™ dye is a DNA modifier used for viability PCR (v-PCR). V-PCR is a powerful PCR-based, culture-independent, method for rapid and sensitive quantification of microbial viability.
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Propidium iodide 1 mg/ml in water
Supplier: Biotium
Propidium iodide (PI) is a membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a cell population and also used as a counterstain in multicolor fluorescent imaging.



