Supplier: AGILENT

The AriaMx real-time PCR system is a fully integrated quantitative PCR amplification, detection and data analysis system.

Supplier: DOEHLER GROUP

PCR broth for fast and universal detection of beer spoiling microorganisms, such as lactobacilli, pediococci, Pectinatus and Megasphaera in yeast and clear/cloudy beer samples. NBB®-PCR (pH 5,7 ±0,2) is a ready-to-use enrichment broth for the detection of beer spoiling bacteria by PCR and real-time PCR methods. NBB®-PCR is compatible with all standard PCR and real-time PCR methods from different manufacturers. Due to an optimised and regulated quality-controlled production process, the medium does not contain any ingredients, which impair DNA amplification and detection (realtime PCR). In addition the medium is free from contaminating DNA, which is controlled and ensured by internal and external validations. The medium is optimized for microbiological enrichment and therefore allows no culture detection of bacteria, because no indicator dye is included.
NBB®-PCR broth can be used for the molecular biological detection of:.

Supplier: AGILENT

Detects low copy number target and withstands PCR inhibitors without extensive purification.

Supplier: AGILENT

Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.

Supplier: AGILENT

PfuUltra High-Fidelity DNA Polymerase AD features a reformulated buffer system for increased economy with the same high performance as the original PfuUltra DNA Polymerase for robust, ultra-high-fidelity PCR.

Supplier: VWR Collection

VWR®, PCR tubes, Cap strip, Single sheet/mat/strip, Optically clear 8-cap strips for Real-Time PCR plates, PP

Supplier: AGILENT

Brilliant III Ultra-Fast SYBR® QPCR and QRT-PCR Master Mixes deliver superior specificity by utilizing a novel faster-activating hot start method to minimize the formation of primer-dimers and other off-target reactions.

Supplier: Thermo Fisher Scientific

Piko® PCR plates utilise ultra-thin wall (UTW) technology to dramatically reduce the thickness of the well walls, and are specifically designed for use in the Piko® thermal cycler and the PikoReal® Real Time PCR system. Piko® PCR plates have the footprint of a microscope slide. Four Piko® 24-well (96-well) plates can be snapped into the plate frame to create a standard 96-well (384-well) plate.

Supplier: Quantabio

Q qPCR cycler uses a patented magnetic induction technology to rapidly heat samples, coupled with fan-forced air for cooling to acquire data in only 25 minutes. Available as four channel models, the robust optical system acquires all channels simultaneously and allows for running the fastest multiplexed assays.

Supplier: AGILENT

High-throughput PCR fragment sizing and microsatellite detection.

Supplier: AGILENT

DNA fragment analysis for PCR, genotyping, and microsatellite applications.

Supplier: Corning

The efficient removal of unincorporated dNTPs, salts and enzymes after any PCR is detrimental to the success or failure of downstream applications, such as genotyping, Sanger, and Next Generation Sequencing. The AxyPrep™ Mag PCR Clean Up Kit utilises a paramagnetic bead technology for rapid high throughput purification of PCR products. Additionally, the AxyPrep™ Mag PCR Clean Up Kit employs an optimised buffer to selectively bind PCR amplicons 60 bp and larger to paramagnetic beads, providing end users flexibility and high recovery options. The protocol comprises binding, washing and eluting steps, where primers, nucleotides, salts and enzymes in the reaction mixture are efficiently removed leaving a purified PCR product that is essentially free of contaminants. No centrifugation or filtration steps are required, allowing the process to be adapted to multiple commercially available automation platforms.

Supplier: Quantabio

This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.

Supplier: VWR Collection

These PCR tube strips and caps are made of PP resin and are designed to fit most popular brands of thermal cyclers.

Supplier: OMEGA BIO-TEK

The E-Z 96® Plant DNA DS Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols, or those having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Plant DNA DS Mini Kit is designed for efficient recovery of genomic DNA up to 30 kb in size from fresh, frozen, or dried plant tissue samples rich in polysaccharides, polyphenols or having a lower DNA content. Up to 50 mg wet tissue can be processed in less than 1 hour. The system combines the reversible nucleic acid-binding properties of the HiBind® matrix with the speed and versatility of spin column technology to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. Purified DNA is suitable for PCR, restriction digestion, and hybridisation applications.

Supplier: OMEGA BIO-TEK

The Mag-Bind® Endo-Free plasmid 96 kit combines the power of Mag-Bind® technology with the innovative ETR technology to deliver high quality endotoxin-free plasmid DNA in a high throughput format. By adding a quick endotoxin removal step, the Mag-Bind® Endo-Free plasmid 96 kit provides a flexible and fast solution for isolating endotoxin free plasmid on a small economical scale. The flexible system can be easily automated, thereby making it an ideal option for high throughput transfection screening as well as for other standard molecular biology techniques including restriction enzyme digestion.

Supplier: VWR Chemicals

The peqGOLD DNase I Digest Kit is optimised for use with peqGOLD RNA extraction protocols. Normally DNase I digestion is not required for RNA purified with peqGOLD RNA Mini Columns as our silica-based spin column technology efficiently removes the majority of DNA without enzymatic digestion. However, certain sensitive RNA applications may require further DNA removal.

Supplier: Corning

The AxyPrep™ FragmentSelect-I Kit is optimized for Illumina next generation sequencing platforms and Life Technologies (SOLiD) fragment size selection needs to simplify the library construction workflow. The kit is designed for fragment size selection needs in the Roche/454’s DNA Genome sequencer library construction workflow.

Supplier: VWR Chemicals

Isolate up to 40 to 70 µg of high quality plasmid DNA from up to 15 ml bacterial cultures in 30 minutes or less. Simplified with VWR® peqGold Mini Column II technology into three quick steps: Bind, wash and elute.

Supplier: AGILENT

StrataClean resin eliminates the need to perform phenol:chloroform extractions, which are highly toxic and combustible.

Supplier: AGILENT

Absolutely RNA Product portfolio makes RNA purification from tissue and cell samples easy and fast.

Supplier: AGILENT

RNA isolation kits provide a straightforward, spin-column method to deliver RNA for use in gene expression and other downstream analysis.

Supplier: VWR Chemicals

Real-Time PCR kit for detection and quantification of Legionella spp. in environmental samples (ISO/TS 12869). Kit contains all necessary reagents for 100 reactions. This product provides rapid and efficient detection and quantification of Legionella spp.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® Stool DNA kit allows rapid and reliable isolation of high quality total DNA from fresh and frozen stool samples. Up to 200 mg of stool samples can be processed in less than 60 minutes. The system combines the reversible nucleic acid-binding properties of HiBind matrix with the speed and versatility of spin column technology to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from stool samples. Purified DNA is suitable for PCR, restriction digestion, and hybridisation techniques.

Supplier: OMEGA BIO-TEK

E.Z.N.A.® Cycle-Pure kits are designed for the rapid purification of single or double-stranded DNA from PCR or other enzymatic reactions. The purification procedure completely removes primers, nucleotides, enzymes, salts, and other impurities from DNA samples. E.Z.N.A.® MicroElute Cycle-Pure kits are specifically intended to purify PCR samples with a small elution volume of 10 to 15 µl. The E-Z® 96 Cycle-Pure kit procedure allows for the parallel purification of up to 96 PCR samples from multiple amplifications. The E-Z® 96 Cycle-Pure kit utilises multiwell technology for manual or fully automated high throughput purification.

Supplier: AGILENT

High-quality well plate supplies and accessories will enhance your PCR and qPCR workflow and analysis.

Supplier: AGILENT HEWLETT PACKARD

CE/MS sprayer kit and CE/MS adapter kit to couple an Agilent CE instrument with an MS system.

Supplier: AGILENT

The RecoverEase DNA isolation kit recovers high-molecular-weight (100 to 500 kb) genomic DNA that is fully hydrated and ready for multiple applications the next day. DNA can be isolated from whole tissues such as liver, spleen, kidney, brain and testes without hazardous and tedious organic extractions or ethanol precipitations. The RecoverEase DNA isolation kit quickly isolates high-molecular-weight genomic DNA from a variety of tissues without organic solvent extractions or ethanol precipitation.

Supplier: AGILENT

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.