Vectors, pBlueScript II
pBluescript II SK(+) Phagemid Kit, f1 origin in (+) orientation, Sac-Kpn polylinker orientation, contains: 20 μg pBluescript II SK(+) phagemid vector, host strain: XL1-Blue MRF', 1 kt
Catalog # AGLS212205
Supplier: AGILENT
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Product Details & Documents
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
- High copy number ColE1-based phagemid
- Large and versatile polylinker in two orientations, f1 origin available in either (+) or (–) orientation
- T3 and T7 promoters for in vitro transcription of RNA
The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.
: For research use only. Not for use in diagnostic procedures.
Specifications
Specifications
- Pk:1 kt
- Description:pBluescript II SK(+) Phagemid Kit, f1 origin in (+) orientation, Sac-Kpn polylinker orientation, contains: 20 μg pBluescript II SK(+) phagemid vector, host strain: XL1-Blue MRF'
Specifications
Methanol ≥99.8%, HiPerSolv CHROMANORM® Reag. Ph. Eur., gradient grade for HPLC
#: 20864.320
Supplier: VWR Chemicals
Acetonitrile ≥99.9%, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments
#: 83640.320
Supplier: VWR Chemicals
Acetonitrile ≥99.9%, LiChrosolv® Reag. Ph. Eur., gradient grade for liquid chromatography, Supelco®
#: 1.00030.2500
Supplier: Merck
Sodium hydroxide 0.1 mol/l (0.1 N) in aqueous solution, AVS TITRINORM Reag. Ph. Eur. volumetric solution
#: 31770.294
Supplier: VWR Chemicals
Water, HiPerSolv CHROMANORM® for LC-MS, suitable for UPLC/UHPLC instruments
#: 83645.320
Supplier: VWR Chemicals
Buffer solutions, ready to use, referenced at 20 °C, Certipur®, Supelco®
#: 1.09439.1000
Supplier: Merck