The NEXTFLEX™ RNA-sequence 2.0 UDI barcodes are designed to prepare multiplexed single and paired-end cDNA libraries from total RNA, mRNA-enriched RNA or rRNA-depleted RNA for Illumina platforms.

• UDI barcodes for multiplexing RNA-sequence libraries Compatible with both paired-end and single-read sequencing Every lot is tested for index purity and functionality by sequencing

The NEXTFLEX™ unique dual index barcodes contain two unique indexes on each adapter, with a hamming distance of at least three throughout the entire series, to allow error correction. The NEXTFLEX™ unique dual index RNA-sequence barcodes can be used for PCR-free RNA sequencing applications. Patterned flow cells found on the Illumina® Novasequence®, Hisequence® 3000/4000, and Hisequence® X platforms suffer from increased rates of sample mis-assignment during sequencing. The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. Every lot is tested for index purity and functionality by sequencing.