Specifications
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Primary antibody reactivity:Other
- Target protein:GLP-1
- Description:GLP-1 ELISA kit
- Sample type:Serum, plasma, tissue homogenates, and other biological fluids
- Cross reactivity:GLP-1 ELISA Kit exhibits high specificity and excellent specificity for the detection of universal GLP-1. No significant cross-reactivity or interference between GLP-1 and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 hours
- Shelf life:Store for 6 months at 4 °C
- Detection range:31,25 - 2000 pmol/L
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:18,75 pmol/L
- Regulatory status:RUO
- Tests per kit:96 tests
Specifications
About this item
GLP-1 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of GLP-1 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-to-use ELISA kit
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
GLP-1 ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of GLP-1 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for GLP-1 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the GLP-1 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-GLP-1 Antibody, which binds the captured GLP-1 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of GLP-1 captured in each well. The concentration of GLP-1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.