qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells.

Quickly and cost-effectively detect the 8 most common karyotypic abnormalities reported in human pluripotent stem cells (hPSCs).

This qPCR-based kit enables the genetic screening of multiple hPSC lines and contains enough material to analyse 20 individual samples in triplicate. It uses double-quenched probes with a 5-carboxyfluorescein (5-FAM) dye to give superior performance over other, single-quenched probes. The kit includes qPCR Master Mix, ROX Reference Dye, Buffer, and primer/probe mixes designed to detect the critical minimal regions of the 8 most commonly mutated regions, as well as a primer/probe mix to a control location in the genome. A genomic DNA control is also provided with demonstrated normal copy number over the regions of interest.

To understand your results, input qPCR data into our online Genetic Analysis App, which will perform statistical analyses, assist with data interpretation, and provide visual representation of the data.