Human Aggrecan ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Aggrecan in serum, plasma, tissue homogenates, and other biological fluids.

• Ready to use ELISA kit
• Detection range: 0,781 to 50 ng/ml
• Sensitivity: 0,469 ng/ml
• Sample type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Human Aggrecan employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Aggrecan in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Aggrecan has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Aggrecan present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Aggrecan Antibody, which binds the captured Aggrecan present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Aggrecan captured in each well. The concentration of Aggrecan can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.