Human Dio3 ELISA kit is a 90 minutes sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Dio3 in serum, plasma, and other biological fluids.

• Higher throughput: Get results in just 90 minutess, with a single wash step
• Detection range: 2,5 to 40 ng/ml
• Sensitivity: 0,098 ng/ml
• Sample Type: Serum, plasma or other biological fluids
• Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Human Dio3 ELISA kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Dio3 in serum, plasma or other biological fluids. An antibody specific for Dio3 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Dio3 present in each sample is bound to the wells by the immobilised antibody. Biotinylated anti-Dio3 antibody, which also binds the Dio3 present in each sample, and Streptavidin-HRP, which binds the Biotinylated anti-Dio3 antibody, are added and the microtiter plate is incubated. Following incubation, unbound Biotinylated anti-Dio3 antibody and unbound Streptavidin-HRP are removed by washing, and two substrate solutions are added to the wells. colour develops in proportion to the amount of Dio3 captured in each well. The colour development is stopped by addition of stop solution which changes the colour from blue to yellow and the intensity of the colour is then measured. The concentration of Dio3 in the samples can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.