Specifications
- Pk:96 Tests
- Assay duration:Multiple steps
- Assay Type:Sandwich
- Format:Pre-coated
- Host:
- Primary antibody reactivity:Human
- Target protein:Necdin
- Description:Human necdin ELISA kit
- Environmentally Preferable:
- Sample type:Serum, plasma, tissue homogenates and other biological fluids
- Cross reactivity:Human Necdin ELISA kit exhibits high specificity and excellent specificity for the detection of human Necdin. No significant cross-reactivity or interference between Necdin and analogues was observed.
- Detection method:Colorimetric
- Time to Results:4 h 30 min
- Assay Principle:Quantitative
- Shelf life:Store for 6 months at 4 °C
- Detection range:0,313 - 20 ng/ml
- Storage temperature:4 °C
- Sample volume:100 μl
- Sensitivity:0,188 ng/ml
- Regulatory status:RUO
Specifications
About this item
Human Necdin ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of human Necdin in serum, plasma, tissue homogenates, and other biological fluids.
- Ready-To-Use ELISA Kit
- Detection Range: 0,313 - 20 ng/ml
- Sensitivity: 0,188 ng/ml
- Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
- Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%
Human Necdin ELISA kit (A79554) employs the sandwich enzyme immunoassay technique for the quantitative measurement of human Necdin in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Necdin has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Necdin present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Necdin Antibody, which binds the captured Necdin present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Necdin captured in each well. The concentration of Necdin can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.