Human AMPK alpha 1 ELISA kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of human AMPK alpha 1 in serum, plasma, tissue homogenates, and other biological fluids.

• Ready to use ELISA kit
• Detection range: 78,125 to 5000 pg/ml
• Sensitivity: 46,875 pg/ml
• Sample type: Serum, plasma, tissue homogenates, and other biological fluids
• Assay precision: Intra assay: CV <8%, Inter assay: CV <10%

Human AMPK alpha 1 ELISA Kit (A75748) employs the competitive enzyme immunoassay technique for the quantitative measurement of human AMPK alpha 1 in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with AMPK alpha 1 antigen. During the incubation, AMPK alpha 1 present in the samples or standards competes with the fixed amount of immobilised AMPK alpha 1 for binding sites on the Biotinylated Anti-AMPK alpha 1 Antibody. The more AMPK alpha 1 present in a sample or standard, the less Biotinylated Anti-AMPK alpha 1 Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-AMPK alpha 1 Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of AMPK alpha 1 present in each sample or standard. The concentration of AMPK alpha 1 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.