Human Recombinant DFF45 (from E. coli)
Catalog # PRSI92-051
Supplier: ProSci Inc.
CAS Number:
Specifications
- Pk:0,05 mg
- Protein/peptide type:Recombinant
- Source:E. coli
- Species:Human
- Tag sequence:N-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimise freeze-thaw cycles.
- Endotoxin content:<0,1 ng/µg (1 IEU/µg) as determined by LAL test
- Protein synonyms:H13|DFF-45|Inhibitor of CAD|DFFA|ICAD|DNA Fragmentation Factor 45 kDa Subunit|DFF1|DNA Fragmentation Factor Subunit Alpha|DFF45
- Protein/peptide name:DFF45
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:38,7 kDa
- Sequence:Met1-Thr331
- Formulation:Supplied as a 0,2 µm filtered solution of 20 mM PB, 150 mM NaCl, pH 7,4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 µg/ml. Dissolve the Lyophilised protein in ddH₂O. Please aliquot the reconstituted solution to minimise freeze-thaw cycles.
- Tested applications:Used for biological assays
Specifications
About this item
DNA Fragmentation Factor Subunit Alpha (DFFA). DFFA exists as a heterodimer (DFF) with DFFB. DFF is activated once DFFA is cleaved by Caspase-3. The cleaved fragments of DFFA detach from DFFB (the active component of DFF), which in turn triggers DNA fragmentation as well as chromatin condensation during apoptosis. A reduced level of DFFA detected in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression.
- Fusion-Tag: N-6 His tag
This recombinant protein can be used for biological assays.
Caution: For research use only.