- Antibody type:Primary
- Antigen name:Midline-1
- Clonality:Polyclonal
- Conjugation:Alexa Fluor® 750
- Host:Rabbit
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- Isotype:IgG
- Reactivity:Human,Rat,Mouse
- Western blot:Yes
- Environmentally Preferable:
- Epitope:171-270/667
- Form:Liquid
- Gene ID:4281
- Antigen synonyms:Midline 1 Opitz/BBB syndrome|GBBB1|ZNFXY|Midline 1|Midline-1|OSX|Midline 1 RING finger protein|Mid1|Tripartite mot protein TRIM18|Tripartite mot containing protein 18|Finger on X and Y mouse homolog of antibody|XPRF|BBBG1|TRI18_HUMAN|Midline 1 ring finger|TRIM18|Tripartite mot-containing protein 18|Midin|TRI18|Midline1|Putative transcription factor XPRF|OGS1|Zinc finger X and Y antibody|RING finger protein 59|RNF59|MID-1|FXY
- Modification:Unmodified
- Storage buffer:Aqueous buffered solution containing 0,01 M TBS (pH 7,4) with 1% BSA, 0,03% Proclin300 and 50% Glycerol
- Storage temperature:Store at ‒20 °C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Concentration:1 µg/µl
- Shipping temperature:4 °C
- Immunogen:KLH conjugated synthetic peptide derived from human Midline-1/RNF59
- Purification:Purified by Protein A
- Pack type:Vial
- Pk:100 µl
Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1. Midline-1 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-1 is believed to have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Defects in MID1 are the cause of Opitz syndrome type I (OS-I). OS-I is an X-linked recessive disorder characterised by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects. OS-I mutations produce proteins with a decreased affinity for microtubules.
Type: Primary
Antigen: Midline-1
Clonality: Polyclonal
Clone:
Conjugation: ALEXA FLUOR® 750
Public Immunogen Range: 171-270/667
Host: Rabbit
Isotype: IgG
Reactivity: Human; Mouse; Rat