A competitive assay for used for quantification of CysLT.

• Detect all CysLTs (LTC₄, LTD₄ and LTE₄) in urine and other sample matrices
• Assay 24 samples in triplicate or 36 samples in duplicate
• Measure CysLT levels down to 40,5 pg/mL
• Incubation: 18 hours | Development: 90 to 120 minutes | Read: Colorimetric at 405 to 420 nm

Leukotrienes (LTs) are a group of acute inflammatory mediators derived from arachidonic acid via the 5-lipoxygenase (5-LO) pathway in leukocytes. LTC₄, LTD₄, and LTE₄ are collectively referred to as cysteinyl leukotrienes (CysLTs). LTC₄ and LTD₄ are potent mediators of asthma and hypersensitivity. They induce bronchoconstriction, increase microvascular permeability, and are vasoconstrictors of coronary arteries. The biological activity of LTE₄ is much lower in most systems studied, but its presence reflects the prior existence of LTC₄ and LTD₄. Cayman's CysLT ELISA Kit is a competitive assay based on a proprietary high-affinity monoclonal antibody that can be used for quantification of CysLT in urine and other sample matrices. The assay has a range from 8,6 to 2500 pg/ml and a sensitivity (80% B/B₀) of approximately 40,5 pg/ml.