The InterPlay Mammalian TAP system allows you to recover interacting proteins from mammalian cells.

• Protease digestion unnecessary to recover intact interacting protein partners since SBP and CBP elute easily from their affinity resins
• Exceptionally pure protein for downstream analysis

This method is based on expression of a protein of interest fused to two affinity tags: a streptavidin binding peptide (SBP) and a calmodulin binding peptide (CBP). Tandem affinity purification yields your tagged protein and interacting proteins using gentle washing and small molecule elution conditions.