Revolutionary technology allows antibody labelling with Cyanine 555 (Cy®3) or Cyanine 647 (Cy®5) in 30 minutes without a purification step. The labelling procedure tolerates many common buffer components and antibody stabilisers.

• Label 5 to 20 µg, 20 to 50 µg, or 50 to 100 µg Ab
• Less than 30 seconds of hands-on time
• 30 minutes total reaction time
• No purification, 100% recovery
• Compatible with BSA, gelatin, ascites
• Choose Cyanine 555 (identical to Cy®3) or Cyanine 647 (identical to Cy®5)

Mix-n-Stain™ Cyanine Dye antibody labelling kits allow you to label ≤5 up to 100 µg of your antibody in just 30 minutes, with minimal hands-on time and no purification.

Mix-n-Stain™ labeling tolerates common antibody buffer formulations. An ultrafiltration centrifuge vial is provided to quickly remove interfering substances like glycerol, or to concentrate your antibody. With a slight modification in protocol, antibodies can be labeled in the presence of BSA, gelatin, or ascites fluid. The presence of other proteins like BSA or gelatin in the labeling reaction has minimal effect on background fluorescence, because any labeled non-antibody proteins readily wash away during immunofluorescence staining.

Biotium offers Mix-n-Stain™ kits for labeling antibodies, nanobodies, and small ligands with a wide selection of fluorescent dyes, enzymes, and other labels.

Mix-n-Stain™ antibody labeling kits are very simple to use (see the workflow overview above). But before you begin, you must check that your antibody meets the compatibility requirements for labeling, and choose the right labeling protocol. Download the product protocol for a labeling check list to help you select the right kit size and labeling protocol for your antibody. Also see the kit compatibility and protocol selection flowchart.

Kit components: Ultrafiltration spin vial (for antibody concentration or buffer exchange, if needed), 10× Mix-n-Stain™ reaction buffer, lyophilized reactive dye, Mix-n-Stain™ storage buffer.