Vectors, pBlueScript II
About this item
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
- High copy number ColE1-based phagemid
- Large and versatile polylinker in two orientations, f1 origin available in either (+) or (–) orientation
- T3 and T7 promoters for in vitro transcription of RNA
The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.
Caution: For research use only. Not for use in diagnostic procedures.
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Product Details & Documents
The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping.
- High copy number ColE1-based phagemid
- Large and versatile polylinker in two orientations, f1 origin available in either (+) or (–) orientation
- T3 and T7 promoters for in vitro transcription of RNA
The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.
Caution: For research use only. Not for use in diagnostic procedures.
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Specifications
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- AGLS200251VCSM13 interference resistant helper phage, Interference-resistant helper phage (6 kB, single stranded). Derived from M13 K07 mutant. Carries kanamycin-resistance gene in IG region. High rescue efficiencies with kanamycin selection, size: 1 mlVCSM13 interference resistant helper phage, Interference-resistant helper phage (6 kB, single stranded). Derived from M13 K07 mutant. Carries kanamycin-resistance gene in IG region. High rescue efficiencies with kanamycin selection, size: 1 ml1Log in for availability1 * 1 STSFr. 412.00Specifications:
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Cat. No.AGLS200251 - AGLS212205pBluescript II SK(+) Phagemid Kit, f1 origin in (+) orientation, Sac-Kpn polylinker orientation, contains: 20 μg pBluescript II SK(+) phagemid vector, host strain: XL1-Blue MRF'pBluescript II SK(+) Phagemid Kit, f1 origin in (+) orientation, Sac-Kpn polylinker orientation, contains: 20 μg pBluescript II SK(+) phagemid vector, host strain: XL1-Blue MRF'1 ktLog in for availability1 * 1 ktSFr. 768.00Specifications:
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Cat. No.AGLS212205 - AGLS212207pBluescript II KS(+) Phagemid Kit, f1 origin in (+) orientation, Kpn-Sac polylinker orientation, contains: 20 μg pBluescript II KS(+) phagemid vector, host strain: XL1-Blue MRF'pBluescript II KS(+) Phagemid Kit, f1 origin in (+) orientation, Kpn-Sac polylinker orientation, contains: 20 μg pBluescript II KS(+) phagemid vector, host strain: XL1-Blue MRF'1 ktLog in for availability1 * 1 ktSFr. 783.00Specifications:
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Specifications
Cat. No.AGLS212207
#: 20864.320
Supplier: VWR Chemicals
#: 83640.320
Supplier: VWR Chemicals
#: 1.00030.2500
Supplier: Merck
#: 31770.294
Supplier: VWR Chemicals
#: 83645.320
Supplier: VWR Chemicals
#: 1.09439.1000
Supplier: Merck