Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation.; Measured by its binding ability in a functional ELISA. Immobilised Biotinylated Human VSIG3, Fc,Avitag at 5 μg/ml (100 μl/well) on streptavidin precoated (0,5 μg/well) plate, can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0,039-0,625 μg/ml (QC tested).; Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin Captured on CM5 chip via anti-mouse antibodies surface can bind Human VSIG3, Fc Tag with an affinity constant of 8,87 μM as determined in a SPR assay (Biacore T200) (Routinely tested).; Loaded Biotinylated Human VSIG3, Fc,Avitag on SA Biosensor, can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 2,6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested)
• Gene ID:
  Q9H7M9-1(D187E)
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  B7-H5|SISP1|Gi24|VISTA
• Protein/peptide name:
  B7-H5
• Purity:
  >95%
• Molecular weight:
  45,0 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT