Unconjugated

Recombinant

HEK293 cells

Human

–20 °C

Less than 1,0 EU per μg by the LAL method.

Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation.; Measured by its binding ability in a functional ELISA. Immobilised Biotinylated Human VSIG3, Fc,Avitag at 5 μg/ml (100 μl/well) on streptavidin precoated (0,5 μg/well) plate, can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0,039-0,625 μg/ml (QC tested).; Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin Captured on CM5 chip via anti-mouse antibodies surface can bind Human VSIG3, Fc Tag with an affinity constant of 8,87 μM as determined in a SPR assay (Biacore T200) (Routinely tested).; Loaded Biotinylated Human VSIG3, Fc,Avitag on SA Biosensor, can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 2,6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested)

Q9H7M9-1(D187E)

Y

Y

Y

B7-H5|SISP1|Gi24|VISTA

B7-H5

>95%

45,0 kDa

Low

Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation

Y

Powder,RT