Human recombinant B7-H5 (from HEK293)
- Conjugation:Unconjugated
- Protein/peptide type:Recombinant
- Source:HEK293 cells
- Species:Human
- Storage conditions:–20 °C
- Endotoxin content:Less than 1,0 EU per μg by the LAL method.
- Biological activity:Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation.; Measured by its binding ability in a functional ELISA. Immobilised Biotinylated Human VSIG3, Fc,Avitag at 5 μg/ml (100 μl/well) on streptavidin precoated (0,5 μg/well) plate, can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0,039-0,625 μg/ml (QC tested).; Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin Captured on CM5 chip via anti-mouse antibodies surface can bind Human VSIG3, Fc Tag with an affinity constant of 8,87 μM as determined in a SPR assay (Biacore T200) (Routinely tested).; Loaded Biotinylated Human VSIG3, Fc,Avitag on SA Biosensor, can bind Human B7-H5, Mouse IgG2a Fc Tag, low endotoxin with an affinity constant of 2,6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested)
- Gene ID:Q9H7M9-1(D187E)
- Carrier-free:Y
- Protease-free:Y
- Animal-free:Y
- Protein synonyms:B7-H5|SISP1|Gi24|VISTA
- Protein/peptide name:B7-H5
- Purity:>95%
- Molecular weight:45,0 kDa
- Endotoxin level:Low
- Formulation:Lyophilised from 0,22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH 7,5. Normally trehalose is added as protectant before lyophilisation
- Nuclease-free:Y
- Shipping temperature:Powder,RT
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