Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 0,1 EU per μg by the LAL method.
• Biological activity:
  Lyopilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.; Measured by its binding ability in a functional ELISA. Immobilised Human TNFSF11, His Tag at 2 μg/ml (100 µl/well) can bind Human RANK, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0,4-13 ng/ml (QC tested).; Captured Human RANK, Mouse IgG2a Fc Tag, low endotoxin on CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11, His Tag with an affinity constant of 0,309 nM as determined in a SPR assay (Biacore T200) (Routinely tested).; Loaded Human RANK, Mouse IgG2a Fc Tag, low endotoxin on AMC Biosensor, can bind Human TNFSF11, His Tag (active trimer) with an affinity constant of 0,409 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  Q9Y6Q6-1
• Carrier-free:
  Y
• Protease-free:
  Y
• Animal-free:
  Y
• Protein synonyms:
  CD265 antigen|TNFRSF11A|ODFR|ODFROSTS|RANK|OFE|OSTS|FEO|LOH18CR1|OPTB7|RANKLOH18CR1|CD265|PDB2|TRANCER|NFKB activator
• Protein/peptide name:
  RANK
• Purity:
  >95%
• Molecular weight:
  47,2 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyopilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.
• Nuclease-free:
  Y
• Shipping temperature:
  Powder,RT