Specifications

• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  His Tag (SPR & BLI verified)
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Immobilised Human CD32a (H167), His Tag (SPR & BLI verified) (Cat. No. CD1-H5223) on CM5 Chip via anti-His antibody, can bind  Rituximab with an affinity constant of 1,45 μM as determined in a SPR assay (Biacore T200) (QC tested). Loaded Human CD32a (H167), His Tag (SPR & BLI verified) (Cat. No. CD1-H5223) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1,30 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  P12318-1
• Protein synonyms:
  CD32|CD32a|IGFR2|FCGR2A1|FCG2 |FCGR2A
• Protein/peptide name:
  Fc gamma RIIA/ CD32a
• Purity:
  >95% as determined by SDS-PAGE.
• Molecular weight:
  21,5 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
• Shipping temperature:
  RT