Unconjugated

Recombinant

HEK293 cells

Human

–20 °C

Less than 0,1 EU per μg by the LAL method.

Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation. Measured by its binding ability in a functional ELISA. Immobilised Human CD30, His Tag (Cat. No. CD0-H5229) at 2 μg/ml (100 μl/well) can bind Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) with a linear range of 1 - 25 ng/ml (QC tested). Loaded Biotinylated Human CD30, Avitag,His Tag (Cat. No. CD0-H82E6) on SA Biosensor, can bind Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) with an affinity constant of 114 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). FACS analysis shows that Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) can bind to K562-CD30 cells surface CD30. The concentration of Human CD30 Ligand is 0,1 μg/ml (Routinely tested). FACS analysis shows that the binding of Human CD30 Ligand, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. CDL-H525b) to K562-CD30 cells surface CD30 was inhibited by increasing concentration of neutralising anti-CD30 antibody. The concentration of Human CD30 Ligand used is 0,1 μg/ml. The IC50 is 0,2064 μg/ml (Routinely tested).

P32971-1

Y

Y

Y

CD30 antigen ligand|CD30-L|TNFSF8|CD30 Ligand|CD30LG|CD153|CD30L|CD153 antigen

CD30 Ligand

>95%

46,5 kDa

Low

Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.

Y

Powder, RT