Recombinant NAD Kinase B. subtilis (from E. coli)
Catalog # PRSI90-364
Supplier: ProSci Inc.
CAS Number:
Specifications
- Pk:50 µG
- Enzyme type:Recombinant
- Source:E. coli
- Storage conditions:Stable for at least 6 months after receipt when stored at –80 °C.
- Enzyme name:NAD Kinase B. subtilis
- Enzyme activity:~2.6 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1umol nicotinamide adenine dinucleotide phosphate (NADP) per min.
- Purity:>98% (SDS-PAGE)
- Formulation:Liquid, In 50 mM TRIS-HCl, pH 7.0 containing 150 mM sodium chloride, 1 mM EDTA and 1 mM dithiothreitol.
Specifications
About this item
NAD kinase catalyses the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Bacillus subtilis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Catalytic activity: ATP + NAD+ = ADP + NADP+.
Fusion-Tag: Tag Free
This recombinant proteins is for research use only.