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Human recombinant Isocitrate Dehydrogenase (from E. coli)
Human recombinant Isocitrate Dehydrogenase (from E. coli)
Catalog # PRSI92-626
Supplier:  ProSci Inc.
CAS Number:  
Human recombinant Isocitrate Dehydrogenase (from E. coli)
Catalog # PRSI92-626
Supplier:  ProSci Inc.
CAS Number:  

Specifications

  • Pk:
    50 µG
  • Enzyme type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Tag sequence:
    C-8 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Enzyme name:
    Isocitrate Dehydrogenase
  • Purity:
    >94% as determined by reducing SDS-PAGE. Endotoxin level less than 0,1 ng/ug (1 IEU/ug).
  • Molecular weight:
    48,1 kDa
  • Sequence:
    Met1-Leu414
  • Formulation:
    Supplied as a 0,2 µm filtered solution of 50 mM Tris, 200 mM NaCl, 10% glycerol, pH 8,0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH₂O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Specifications

About this item

Isocitrate Dehydrogenase [NADP] Cytoplasmic (IDH1) belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 exists as a homodimer, binding one magnesium or manganese ion per subunit. Mutations of IDH1 have been shown to cause metaphyseal chondromatosis with aciduria and are involved in the development of glioma. IDH plays a role in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid.

Fusion-Tag: C-8 His tag

This recombinant protein can be used for biological assays. For research use only.