- Pk:50 µG
- Enzyme type:Recombinant
- Species:Mouse
- Tag sequence:C-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:5'-Nucleotidase
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:58,8 kDa
- Sequence:Trp29-Phe550
- Formulation:Supplied as a 0,2 µm filtered solution of 20 mM TrisHCl, 120 mM NaCl,4mM CaCl₂, 20% Glycerol, pH 7,5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH₂O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Biological assays
Mouse CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyses extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signalling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity.CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.