Specifications
- Pk:50 µG
- Enzyme type:Recombinant
- Source:E. coli
- Species:E. coli
- Tag sequence:N-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:beta-Glucuronidase
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:69.9 kD
- Sequence:Met1-Gln603
- Formulation:Supplied as a 0.2 µm filtered solution of PBS,pH 7.4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Biological assays
Specifications
About this item
Beta-D-glucuronidase from E. coli is a highly specific enzyme in hydrolysing glucuronides. Beta-D-glucuronidase (GLUase) activity can be measured as the rate of production of fluorescent methylumbelliferone (MU), resulting from the hydrolysis of the substrate 4-methylumbelliferyl- beta -d-glucuronide (MUGLU), which is an effective and rapid method for detection and verification of E. coli in food, water, and environmental samples. High purity recombinant beta-Glucuronidase is used in research, biochemical enzyme assays and in vitro diagnostic analysis, detecting a wide range of drugs such as opioids, benzodiasepines, steroids, cannabinoids, and others.
Fusion-Tag: N-6 His tag
This recombinant protein can be used for biological assays. For research use only.