Specifications

Pk:
50 µG
Enzyme type:
Recombinant
Source:
E. coli
Species:
Human
Tag sequence:
N-6 His tag
Storage conditions:
Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
Enzyme name:
Glycine N-Methyltransferase
Purity:
>95% as determined by reducing SDS-PAGE
Molecular weight:
34.9 kD
Sequence:
Met1-Asg294
Formulation:
Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Tested applications:
Bioassay

Specifications

About this item

Glycine N-Methyltransferase (GNMT) is a tetrameric cytosolic protein. GNMT catalyses the synthesis of N-methylglycine from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. It can affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine, playing an important role in maintaining normal AdoMet levels. GNMT is highly expressed in liver. As a major folate-binding protein, GNMT takes part in the detoxification pathway. Defects in GNMT are the cause of hypermethioninemia. the patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases.

Fusion-Tag: N-6 His tag

This recombinant protein can be used for biological assays. For research use only.