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Human recombinant sulphamidase (from cells)
Human recombinant sulphamidase (from cells)
Catalog # PRSI91-726
Supplier:  ProSci Inc.
CAS Number:  
Human recombinant sulphamidase (from cells)
Catalog # PRSI91-726
Supplier:  ProSci Inc.
CAS Number:  

Specifications

  • Pk:
    50 µG
  • Enzyme type:
    Recombinant
  • Source:
    Cells
  • Species:
    Human
  • Tag sequence:
    C-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Enzyme name:
    Sulfamidase
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    55.72 kD
  • Sequence:
    Arg21-Leu502
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl,1 mM GaCl₂, 10% Glycerol,pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Bioassay

Specifications

About this item

N-Sulphoglucosamine Sulphohydrolase (SGSH) is an important member of the sulphatase family which is involved in the degradation of heparin sulphate. SGSH binds one calcium ion per subunit as a cofactor. SGSH catalyses N-sulfo-D-glucosamine and H₂O to D-glucosamine and sulphate. SGSH deficiency is result in mucopolysaccharidosis type 3A (MPS3A), a recessive lysosomal storage disease characterised by neurological dysfunction but relatively mild somatic manifestations.

Fusion-Tag: C-6 His tag

This recombinant protein can be used for biological assays. For research use only.