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Human recombinant ribonuclease 3 (from cells)
Human recombinant ribonuclease 3 (from cells)
Catalog # PRSI91-718
Supplier:  ProSci Inc.
CAS Number:  
Human recombinant ribonuclease 3 (from cells)
Catalog # PRSI91-718
Supplier:  ProSci Inc.
CAS Number:  

Specifications

  • Pk:
    50 µG
  • Enzyme type:
    Recombinant
  • Source:
    Cells
  • Species:
    Human
  • Tag sequence:
    C-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Enzyme name:
    Ribonuclease 3
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    16.6 kD
  • Sequence:
    Arg28-Ile160
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl,1 mMDTT, 10% Glycerol,pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Bioassay

Specifications

About this item

Ribonuclease 3 (RNASE3) is a basic protein that is localised to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarisation of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

Fusion-Tag: C-6 His tag

This recombinant protein can be used for biological assays. For research use only.