Human recombinant ribonuclease 3 (from cells)
Catalog # PRSI91-718
Supplier: ProSci Inc.
CAS Number:
Specifications
- Pk:50 µG
- Enzyme type:Recombinant
- Source:Cells
- Species:Human
- Tag sequence:C-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:Ribonuclease 3
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:16.6 kD
- Sequence:Arg28-Ile160
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl,1 mMDTT, 10% Glycerol,pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
Specifications
About this item
Ribonuclease 3 (RNASE3) is a basic protein that is localised to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarisation of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.