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Human recombinant Pyruvate Kinase (from cells)
Human recombinant Pyruvate Kinase (from cells)
Catalog # PRSI91-701
Supplier:  ProSci Inc.
CAS Number:  
Human recombinant Pyruvate Kinase (from cells)
Catalog # PRSI91-701
Supplier:  ProSci Inc.
CAS Number:  

Specifications

  • Pk:
    50 µG
  • Enzyme type:
    Recombinant
  • Source:
    Cells
  • Species:
    Human
  • Tag sequence:
    C-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Enzyme name:
    Pyruvate Kinase
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    62.9 kD
  • Sequence:
    Met1-Ser574
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM Tris-HCl, 1 mM DTT 0.2 M NaCl, 10% glycerol, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    Bioassay

Specifications

About this item

Pyruvate Kinase Isozymes R/L (PKLR) belongs to the pyruvate kinase family, There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver; R is found in red cells; M1 is the main form in muscle, heart and brain; M2 is found in early fetal tissues. PKLR exists as a homotetramer and catalyses the production of phosphoenolpyruvate from pyruvate and ATP. Defects in PKLR are also the cause of pyruvate kinase deficiency of red cells, which is a frequent cause of hereditary non-spherocytic hemolytic anemia.

Fusion-Tag: C-6 His tag

This recombinant protein can be used for biological assays. For research use only.