Human recombinant Pyruvate Kinase (from cells)
Catalog # PRSI91-701
Supplier: ProSci Inc.
CAS Number:
Specifications
- Pk:50 µG
- Enzyme type:Recombinant
- Source:Cells
- Species:Human
- Tag sequence:C-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:Pyruvate Kinase
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:62.9 kD
- Sequence:Met1-Ser574
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM Tris-HCl, 1 mM DTT 0.2 M NaCl, 10% glycerol, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
Specifications
About this item
Pyruvate Kinase Isozymes R/L (PKLR) belongs to the pyruvate kinase family, There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver; R is found in red cells; M1 is the main form in muscle, heart and brain; M2 is found in early fetal tissues. PKLR exists as a homotetramer and catalyses the production of phosphoenolpyruvate from pyruvate and ATP. Defects in PKLR are also the cause of pyruvate kinase deficiency of red cells, which is a frequent cause of hereditary non-spherocytic hemolytic anemia.
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.