Human recombinant Chitotriosidase-1 (from cells)
PRSI91-407
:
- Pk:50 µG
- Enzyme type:Recombinant
- Source:Cells
- Species:Human
- Tag sequence:C-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:Chitotriosidase-1
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:50.43 kD
- Sequence:Ala22-Asn466
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 7.5. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:For Biological Assays
Chitotriosidase-1 (CHIT1) is a glycoprotein that belongs to the Glycosyl Hydrolase 18 family and Chitinase class II subfamily. It is secreted by cultured macrophages and serves to degrade chitin, chitotriose and chitobiose. CHIT1 may participate in the defense against nematodes and other pathogens. It is highly expressed in the plasma of patients with Gaucher's disease type I, which can be used as diagnostic aid and to evaluate the success of treatment that brings levels back to normal. The amino acid sequence of human CHIT1 is 99%, 76%, 75% and 55% identical to that of chimpansee, rat, mouse and fruit fly, respectively.
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.