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Human recombinant Sentrin-specific protease 7 (from E. coli)
Human recombinant Sentrin-specific protease 7 (from E. coli)
Catalog # PRSI91-151
Supplier:  ProSci Inc.
CAS Number:  
Human recombinant Sentrin-specific protease 7 (from E. coli)
Catalog # PRSI91-151
Supplier:  ProSci Inc.
CAS Number:  

Specifications

  • Pk:
    50 µG
  • Enzyme type:
    Recombinant
  • Source:
    E. coli
  • Species:
    Human
  • Tag sequence:
    N-6 His tag
  • Storage conditions:
    Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
  • Enzyme name:
    Sentrin-specific Protease 7
  • Purity:
    >95% as determined by reducing SDS-PAGE
  • Molecular weight:
    27.3 kD
  • Sequence:
    Met695-Ala864
  • Formulation:
    Supplied as a 0.2 µm filtered solution of 20 mM HEPES, 5% Glycerol, pH 7.4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
  • Tested applications:
    For Biological assays

Specifications

About this item

Sentrin-Specific Protease 7 (SENP7) acts as a SUMO-2/3-specific protease. SENP7 is likely to regulate the metabolism of poly-SUMO-2/3 rather than SUMO-1 conjugation in vivo. SENP7 has a restricted substrate specificity, and displaying paralogue-specific isopeptidase activity. The C-terminal catalytic domain of SENP7 depolymerised poly-SUMO-2 chains but does not have activity against poly-SUMO-1 chains. SENP7 also had isopeptidase activity against di-SUMO-2- and SUMO-2-modified RanGAP1 (Ran GTPase-activating protein 1) but had limited activity against SUMO-1-modified RanGAP1.

Fusion-Tag: N-6 His tag

This recombinant protein can be used for biological assays. For research use only.