Human recombinant Sentrin-specific protease 7 (from E. coli)
Catalog # PRSI91-151
Supplier: ProSci Inc.
CAS Number:
Specifications
- Pk:50 µG
- Enzyme type:Recombinant
- Source:E. coli
- Species:Human
- Tag sequence:N-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:Sentrin-specific Protease 7
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:27.3 kD
- Sequence:Met695-Ala864
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM HEPES, 5% Glycerol, pH 7.4. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:For Biological assays
Specifications
About this item
Sentrin-Specific Protease 7 (SENP7) acts as a SUMO-2/3-specific protease. SENP7 is likely to regulate the metabolism of poly-SUMO-2/3 rather than SUMO-1 conjugation in vivo. SENP7 has a restricted substrate specificity, and displaying paralogue-specific isopeptidase activity. The C-terminal catalytic domain of SENP7 depolymerised poly-SUMO-2 chains but does not have activity against poly-SUMO-1 chains. SENP7 also had isopeptidase activity against di-SUMO-2- and SUMO-2-modified RanGAP1 (Ran GTPase-activating protein 1) but had limited activity against SUMO-1-modified RanGAP1.
Fusion-Tag: N-6 His tag
This recombinant protein can be used for biological assays. For research use only.