Human Recombinant DNA Polymerase beta (from E. coli)
Catalog # PRSI91-139
Supplier: ProSci Inc.
CAS Number:
Specifications
- Pk:50 µG
- Enzyme type:Recombinant
- Source:E. coli
- Species:Human
- Tag sequence:C-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:DNA Polymerase beta
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:39.23 kD
- Sequence:Ser2-Glu335
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, pH 7.8. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
Specifications
About this item
Human DNA polymerase beta is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase beta .
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.