- Pk:50 µG
- Enzyme type:Recombinant
- Source:E. coli
- Species:Human
- Tag sequence:C-6 His tag
- Storage conditions:Store at −20 °C, stable for 6 months after receipt. Please minimize freeze−thaw cycles.
- Enzyme name:Carbonic Anhydrase 5B
- Purity:>95% as determined by reducing SDS-PAGE
- Molecular weight:33.77 kD
- Sequence:Cys34-Pro317
- Formulation:Supplied as a 0.2 µm filtered solution of 20 mM TrisHCl, 100 mM NaCl, pH 8.0. Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 ug/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- Tested applications:Bioassay
Carbonic Anhydrase 5B (CA5B) is a member of alpha-carbonic anhydrase family (CAs) that catalyze the reversible hydration of carbon dioxide. CAs is associated with many biological processes, including calcification, respiration, bone resorption, acid-base balance and the formation of aqueous humor. CA5B is highly expressed in heart, pancreas, kidney, placenta, lung, and skeletal muscle, but it is restricted to the liver. CA5B is localised in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA, CA-VA. CA5B is inhibited by coumarins, sulfonamide derivatives such as acetazolamide (AZA), saccharin, and Foscarnet.
Fusion-Tag: C-6 His tag
This recombinant protein can be used for biological assays. For research use only.