Glycerol-3-phosphate oxidase has been used for sensitive metabolite assays of starch and lipid synthesis, pyrophosphate, ATP, ADP, and most glycolytic intermediates in Arabidopsis seeds. It is a part of the dihydroxyacetone phosphate:glycerol-3-phosphate cycle in the bloodstream form of Trypanosoma brucei.

• Soluble in cold 20 mM Tris HCl, pH 7,5 at 37 °C, containing 0,2% (w/v) BSA
• Enzyme Commission Number: E.C.1.1.3.21

It catalyses the conversion of sn-glycerol-3-phosphate to glycerone phosphate and peroxide.

Unit definition: One unit will oxidise 1,0 µmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H₂O₂ per min at 37 °C.