E. coli

Pufferfish

Lyophilized Pufferfish Leptin although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution Leptin should be stored at 4 °C between 2-7 days and for future use below −18 °C. For long term storage it is recommended to add carrier protein (0. 1% HSA or BSA). Please prevent freeze/thaw cycles.

Biological active as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. The affinity of human leptin receptors is considerably lower campared to mammalian leptins.

LEP

Pufferfish Leptin

Greater than 99.0% as determined by RP-HPLC and analysis by SDS-PAGE

It is recommended to reconstitute the lyophilized Pufferfish Leptin in sterile 0.4% NaHCO3 pH-9 not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Leu-Pro-Gly-Ala.

The Pufferfish Leptin was lyophilized from concentrated (0.85mg/ml) solution with 0.003mM NaHCO3.

SDS-PAGE