E. coli

Mouse

Lyophilized NGF2 although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution NGF-2 should be stored at 4 °C between 2-7 days and for future use below −18 °C. For long term storage it is recommended to add carrier protein (0. 1% HSA or BSA). Please prevent freeze/thaw cycles.

The ED50 as determined by the dose-dependant induction of choline acetyl transferase in rat basal forebrain primary septal culture was found between 20-50 ng/ml.

Nerve growth factor-2

Neurotrophin 3

Greater than 97.0% as determined by RP-HPLC and analysis by SDS-PAGE

It is recommended to reconstitute the lyophilized Neurotrophin-3 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

The sequence of the first five N-terminal amino acids was determined and was found to be Tyr-Ala-Glu-His-Lys.

Lyophilized from 0.02% TFA.

SDS-PAGE