S. cerevisiae

Human

Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for weeks, should be stored desiccated below −18 °C. Upon reconstitution PDGF BB should be stored at 4 °C between 2-7 days and for future use below −18 °C. Please prevent freeze/thaw cycles.

The ED50, calculated by the dose-dependant proliferation of BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is 1 ng/ml, corresponding to Specific Activity of MIU/mg.

PDGF B, Yeast

Greater than 97.0% as determined by RP-HPLC and analysis by SDS-PAGE

It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Leu-Gly-Ser-Leu.

The protein was lyophilized from 0.2 um filtered solution in 20 mM sodium acetate in absence of any carrier protein.

SDS-PAGE