Specifications

• Source:
  E. coli
• Species:
  Rat
• Storage conditions:
  Aliquot and store at −20 °C or −80 °C. Avoid repeated freeze/thaw cycles.
• Biological activity:
  ED50 1.0-10.0 ng/mL. The biological activity was determined by measuring the dose dependent chemotaxis with human THP-1 cells. The optimal concentration should be determined for each specific application by an initial dose-response assay.
• Protein synonyms:
  C-C motif chemokine 2
• Protein/peptide name:
  MCP1
• Purity:
  Greater than 95.0% as determined by SDS-PAGE.
• Solubility:
  It is recommended to reconstitute the lyophilized Monocyte Chemotactic Protein-1 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Asp-Ala-Val.
• Formulation:
  The protein was lyophilized from concentrated (1mg/ml) sterile solution containing no additives.
• Tested applications:
  SDS-PAGE , HPLC , FuncS