Specifications

• Source:
  E. coli
• Species:
  Rat
• Storage conditions:
  Store at 4 °C for up to two weeks. For long term storage, aliquot and store at −20 °C, avoid freeze/thaw cycles.
• Biological activity:
  The ED50= 0.03-0.1 ng/mL. The biological activity is determined by measuring the dose-dependant proliferation of IL-6 dapendent B9 cells. concentration range of 0.1 to 10.0 ng/mL is effective for most in vitro applications.
• Protein synonyms:
  Interleukin BSF 2
• Protein/peptide name:
  IL6
• Purity:
  > 95.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Solubility:
  It is recommended to reconstitute the lyophilized Interleukin-6 in 100mM acetic acid to 1.0 mg/mL and incubated for 30 minutes at room temperature to regain full activity, which can then be further diluted to other aqueous solutions.
• Sequence:
  The sequence of the first five N-terminal amino acids was determined and was found to be Met-Phe-Pro-Thr-Ser.
• Formulation:
  The protein was lyophilized without any additives.
• Tested applications:
  SDS-PAGE , HPLC , FuncS