RNA 3' End desthiobiotinylation kit
Catalog # PIER20163
Supplier: Thermo Fisher Scientific
CAS Number:
Specifications
- Pk:1 KIT
- Description:Pierce™ RNA 3' end desthiobiotinylation kit, sufficient for 20 desthiobiotinylation reactions using 50 pmol of RNA each
Specifications
About this item
Pierce™ RNA 3' End Desthiobiotinylation Kit contains reagents for easy and efficient desthiobiotin labeling of RNA for use as probes or as antibody alternatives for enrichment of RNA binding proteins.
- A single biotin labels has similar detection sensitivity as radioactivity
- Results in minimal disturbance of RNA secondary structure
- Desthiobiotin label may be used for detection or as an affinity handle for streptavidin resin
Pierce™ RNA 3' End Desthiobiotinylation Kit is optimised for labeling the 3'-end of single-stranded RNA using T4 RNA ligase. Once labeled, the RNA can be used as a probe or target for gel-shift EMSA reactions, Northern blots and protein-RNA interaction experiments. The desthiobiotinylated RNA also can be used to enrich for RNA binding proteins (RBP) using streptavidin affinity resin.
Delivery: Kit includes desthiobiotinylated cytidine bisphosphate (40 µl), T4 RNA ligase (40 µl), T4 RNA ligase reaction buffer,10X (100 µl), non-labeled RNA control (100 µl), biotinylated IRE RNA control (35 µl), RNase inhibitor (2×10 µl), DMSO (200 µl), PEG 30% (300 µl), glycogen (20 µl) and nuclease-free water (1,5 ml).