The Pierce™ TMB Substrate Kit contains soluble TMB (3,3´,5,5´-tetramethylbenzidine) that is ideal for sensitive ELISA-based applications. TMB is a chromogenic substrate for horseradish peroxidase. Once oxidised by the enzyme, this substrate yields a blue product that absorbs at 370 nm and 652 nm. Adding sulfuric or phosphoric acid to stop the reaction changes the product to yellow that absorbs at 450 nm.

• • Available in traditional or ready-to-use formulations
• • No additional reagents or filtering required
• • Non carcinogenic
• • Various levels of sensitivity and development speed to suit any assay
• • No DMF or DMSO present in the reagents

Substrate type: HRP (Horseradish Peroxidase)
Detection method: Colorimeteric method