QuantaRed™ Enhanced Chemifluorescent HRP Substrate contains chemical enhancers to increase the fluorescent yield and sensitivity of 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) chemifluorescence.

• Low picogram to femtogram detection
• Minimal autofluorescence interference from biological samples
• Use Stop Solution to effectively stop the reaction resulting in stable signal for at least 4 hours
• Produces a strong colorimetric signal and can be read fluorescently or colorimetrically at 570 nm

ADHP is a non-fluorescent compound that reacts with horseradish peroxidase (HRP) to produce resorufin, a soluble, highly fluorescent reaction product with excitation/emission maxima of ~570/585nm. The long wavelength emission of the resorufin minimises interference from the low wavelength (blue and green) autofluorescence, making it an ideal substrate for quantitating target molecules in biological samples.