- Antibody type:Secondary
- Antigen name:Immunoglobulin G
- Antigen symbol:IgG
- Clonality:Polyclonal
- Conjugation:TRITC (Tetramethylrhodamine Isothiocyanate)
- ELISA:Yes
- Flow cytometry:Yes
- Host:Donkey
- ImmunoChemistry:Yes
- ImmunoFluorescence:Yes
- ImmunoPrecipitation:Yes
- Isotype:IgG
- Reactivity:Mouse
- Western blot:Yes
- Cross adsorption:Yes
- Format:Purified IgG
- Form:Lyophilized
- Antibody fragment:F(ab')2
- Storage buffer:10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free
- Storage temperature:2-8 °C
- Concentration:1,0 mg/ml (E 1% at 280 nm = 13,0)
- Shipping temperature:2-8 °C
- Immunogen:Mouse IgG
- Purification:Affinity purified using solid phaseMouse IgG
- Size:0,5 mg
- Pk:0,5 mg
TRITC (Tetramethylrhodamine isothiocyanate) is a popular rhodamine derivative pink in color but emits a red-orange color upon Emission at 580nm. TRITC is frequently paired with FITC in double labeling experiments and conjugated antibodies are often used in immunofluorescence microscopy, flow cytometry, FLISA (fluorescent ELISA) and high throughput screening assays. These conjugates are best excited with the 532 nm spectral line of the He-Ne laser and are also recommended as a second color detection reagent in in situ hybridization applications. (Excitation/Emission = 555nm / 580nm Emission Color = Orange to Red (Similar Dyes: Alexa Fluor 555, Cy3, DyLight 550)
Western blot analysis of recombinant human IL-7 (100-11) using rabbit anti-human IL-7 polyclonal antibody and anti-rabbit secondary antibodies. MW of human IL-7 is 17.5 kDa.
Type: Secondary
Antigen: IgG
Clonality: Polyclonal
Clone:
Conjugation: TRITC (Tetramethylrhodamine Isothiocyanate)
Epitope:
Host: Donkey
Isotype: IgG
Reactivity: Mouse