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You searched for: Molecular Weight Markers

Molecular Weight Markers

Molecular-weight size markers are a set of standards used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix. The molecular weight markers offered are ideal for verification of PCR products, for size analysis of digested DNA, and easy band identification of a number of different fragments. Certain models are available pre-mixed with loading dye.

Prestained protein markers, Peacock™ Plus

Prestained protein markers, Peacock™ Plus

Supplier: Biotium

A visible three- colour protein marker for SDS-PAGE or western blotting, with 12 bands ranging from 8 to 245 kDa.

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DNA markers

DNA markers

Supplier: LONZA

DNA marker is for rapid size estimation of PCR products and restriction fragments. DNA can be visualised by ethidium bromide staining or kinased with radiolabelled ³²P for detection by autoradiography.

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Perfect DNA™ markers

Supplier: Merck Millipore (Novagen)

Molecular size markers for routine use in plasmid cloning, the Perfect DNA™ markers contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities except for the indicated reference bands, which are useful for instant band identification.

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Prestained protein markers, Peacock™

Prestained protein markers, Peacock™

Supplier: Biotium

A visible three-colour protein marker for SDS-PAGE or western blotting, with 10 bands ranging from 10 to 180 kDa.

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DNA ladder, 1 kb Plus, DNAmark™

DNA ladder, 1 kb Plus, DNAmark™

Supplier: G-Biosciences

DNAmark™ 1 kb Plus ladder contains 15 DNA bands ranging from 100 bp to 10 kb. The 500 bp and 3000 bp fragment are present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 µl loading, all fragments except 500 bp and 3000 bp are 40 ng. The 500 bp and 3000 bp fragment are 100 ng.

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Protein markers, ProSieve™ Colour

Protein markers, ProSieve™ Colour

Supplier: LONZA

ProSieve™ Color protein marker have been labelled with three different fluorescent dyes. These markers are supplied as a lyophilised solid containing the buffer salts and detergent found in the typical Laemmli buffer system. It contains nine proteins with approximate masses of 10, 15, 20, 25, 40, 50, 80, 125, and 190 kDa.

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DNA marker, broad range

Supplier: Thermo Fisher Scientific

From 250 to 5000 base pairs: 5 bands from 125 to 1000 bp; four bands from 1500 to 5000 bp. Recommended load volume 20 µl.

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Western blotting markers, Perfect Protein™, Novagen®

Supplier: Merck Millipore (Novagen)

The Perfect Protein™ Western Markers consist of seven recombinant S•Tag™/His•Tag® fusion proteins that can be detected on any Western blot using an S-protein AP or HRP conjugate. As an additional option, the markers can also be detected with an His•Tag Monoclonal Antibody and an AP or HRP conjugated secondary antibody. The Perfect Protein Western Markers serve as precise size standards that appear simultaneously on the blot with target proteins, eliminating the uncertainty and imprecision associated with other methods.

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DNA marker, high range, 1000 bp ladder

Supplier: Thermo Fisher Scientific

DNA ladders are used in gel electrophoresis to determine the size and quantity of testing DNA fragments of genomic, plasmid, and PCR DNA. They are commonly used in the studies of plasmid DNA and PCR products.

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PCR Molecular Size Marker

PCR Molecular Size Marker

Supplier: Merck Millipore (Novagen)

PCR Molecular Size Marker contains a mixture of eight defined DNA fragments ranging from 50 to 2000 bp at convenient size intervals for characterising small DNA products. The markers are supplied in gel loading buffer containing two tracking dyes that do not interfere with UV illumination of ethidium bromide-stained bands. A separate vial of 6X Loading Buffer is included. Each vial of markers is sufficient for 50 lanes on TBE or TAE agarose stained with ethidium bromide. The recommended amount for loading (5 µl) produces bands of even intensity that are bright, sharp and easy to photograph.

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Perfect DNA™ ladders

Supplier: Merck Millipore (Novagen)

The Perfect DNA™ ladders contain sets of DNA species having convenient, easy-to-remember sizes for agarose gel analysis. The markers have uniform band intensities and cover a wide range of DNA sizes.

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DNA ladder, 100 bp

DNA ladder, 100 bp

Supplier: PanReac AppliChem

This DNA ladder is ideal for determining the size of double-stranded DNA ranging from 100 to 1,000 base pairs. The ladder consists of ten fragments that range in size from 100 to 1,000 bp in 100 bp increments. The 500 bp fragment is present at increased intensity to allow easy identification. All fragments are blunt-ended.

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DNA QuantLadders

Supplier: LONZA

QuantLadders are prepared from five highly purified DNA fragments that provide visual quantitation of DNA within ±1% accuracy. The DNA QuantLadder provides each DNA fragment in perfect relative mass equivalence at 100 ng, 70 ng, 50 ng, 20 ng and 10 ng. The reverse QuantLadder simply reverses the mass equivalence order of the QuantLadder.

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Protein markers for gel electrophoresis

Protein markers for gel electrophoresis

Supplier: PanReac AppliChem

The main applications for prestained protein markers are the monitoring of protein migration during SDS-PAGE, verification of Western transfer efficiency on membranes, and estimation of protein sizes on SDS-polyacrylamide gels and Western blots.

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DNA ladder

DNA ladder

Supplier: LONZA

Thse DNA ladders provides rapid and easy size determination of the DNA samples. Each band has an ideal 50 % G-C content for precise sizing and sharp resolution. It is free from contaminating plasmid DNA with minimal background.

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pI markers

pI markers

Supplier: Cytiva

pI markers are lyophilized mixtures of stable, salt-free, highly purified proteins.

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Western blotting markers, Amersham™ ECL™ DualVue™

Western blotting markers, Amersham™ ECL™ DualVue™

Supplier: Cytiva

Amersham™ ECL™ DualVue™ Western blotting markers perform two key functions. Firstly, the prestained marker proteins confirm protein transfer in addition to clearly defining blot orientation. Secondly, the recombinant tagged proteins are detected on film or by CCD imaging in conjunction with the target protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target protein(s) and markers.

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