You searched for: Electrophoresis Reagents
Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.
Fluorogenic peroxidase substrate kit, QuantaBlu™
Supplier: Thermo Fisher Scientific
QuantaBlu™ Fluorogenic peroxidase substrate kit is a three-component kit containing proprietary fluorescent substrate, peroxide and stop solutions for stopped (endpoint) or non-stopped assays.
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Pierce™ Biotin 3' End DNA Labelling Kit
Supplier: Thermo Fisher Scientific
This Biotin 3' End DNA Labelling Kit is used for tagging single-stranded DNA primers with biotin for use in non radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.
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Far-western blot kit for biotinylated proteins, Pierce™
Supplier: Thermo Fisher Scientific
The Pierce™ Far-Western Blot Kit for Biotinylated Proteins enables in-gel or on-membrane Western blot detection of target proteins through interaction with any biotin-labeled test protein used as the primary probe. On-membrane detection offers greater sensitivity whereas in-gel detection is faster and prevents problems associated with incomplete or inefficient transfer.
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Pierce™ Clean-Blot™ IP Detection Kit and Reagents
Supplier: Thermo Fisher Scientific
Clean-Blot™ IP detection kit (HRP) contains enough Clean-Blot™ IP detection reagent and accessory reagents for 25 mini-blots or 2000 cm² of membrane. Clean-Blot™ IP detection reagent is a horseradish peroxidase conjugate that is optimised for post-immunoprecipitation western blot detection of primary antibodies without interference from denatured IP antibody fragments.
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Horseradish peroxidase, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ Horseradish peroxidase (HRP) is purified horseradish peroxidase enzyme for use in activity assays and conjugation to antibodies for ELISA, Western blot and immunohistochemistry applications.
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HRP conjugates, HisProbe™
Supplier: Thermo Fisher Scientific
HisProbe™-HRP is a nickel (Ni²⁺)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.
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Biotin random prime DNA labelling kit, North2South™
Supplier: Thermo Fisher Scientific
North2South™ Biotin Random Prime DNA Labeling Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridisation and detection methods.
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DAB substrate
Supplier: Thermo Fisher Scientific
DAB (3,3'-diaminodbenzidine) is a sensitive colorimetric substrate used with horseradish peroxidase (HRP) in immunohistochemistry and immunoblotting applications. HRP catalyses hydrogen peroxide oxidation of substrates such as DAB. Electrons are transferred by HRP from the DAB to the peroxide to yield an insoluble brown product.
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DNA ladders
Supplier: Biotium
Ready to use DNA ladders are supplied in 1X loading buffer at an optimal concentration for loading GelRed™ and GelGreen™ precast gels.
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Protein ladders (pre-stained), ScanLater™
Supplier: Molecular Devices
The ScanLater™ Western blot protein ladder is a mixture of seven biotinylated recombinant proteins at 10, 20, 40, 60, 80, 100, and 140 kD.
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Prestained Protein Ladder - Mid-range molecular weight (10 - 180 kDa), Abcam
Supplier: Abcam
10-180 kDa prestained protein ladder is a three-color protein standard with 10 pre-stained proteins. - Suitable for western blot (WB), SDS-PAGE - Designed for monitoring protein separation - Supplied in gel loading buffer and is ready to use
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Upstate® Autorad Orientation Markers for Western Blotting
Supplier: Merck
Autorad orientation markers for use on immunoblots in detection of Chemiluminescence in Western blotting, dot/slot blotting.
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SuperSignal™ Pierce™ West Pico PLUS chemiluminescent substrate
Supplier: Thermo Fisher Scientific
SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by Western blot analysis.
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Pierce™ Firefly Signal Enhancer (100X)
Supplier: Thermo Fisher Scientific
Pierce™ Firefly Signal Enhancer (100X) is designed to complement the Firefly Luciferase Flash and Glow assay and is recommended when maximum sensitivity is desired.
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SuperSignal™ Protein ImmunoDetection Reagents, ELISA Femto Maximum Sensitivity Substrate
Supplier: Thermo Fisher Scientific
SuperSignal® ELISA Femto maximum sensitivity substrate utilises the SuperSignal® system with an enhancer formulation for superior protein detection and low-end linearity in ELISA applications. Ideal for automated high-throughput screening applications.
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Western blotting kits, SuperSignal™ west pico chemiluminescent substrate kits, Pierce™
Supplier: Thermo Fisher Scientific
These kits include SuperSignal™ West Pico Substrate that is a highly sensitive enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP) on immunoblots. The kits also contain reagents for the specific detection of biotinylated antibodies, rabbit IgG or mouse IgG. The substrate’s intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using photographic or other imaging methods. Blots can be repeatedly exposed to film to obtain optimal results or stripped of the immunodetection reagents and reprobed.
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Pierce™ OPD (o-phenylenediamine Dihydrochloride)
Supplier: Thermo Fisher Scientific
OPD (o-phenylenediamine dihydrochloride) is a water-soluble substrate for horseradish peroxidase (HRP) that produces a yellow-orange product detectable at 492 nm by ELISA plate readers.
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Antibody extender solution NC, Miser™
Supplier: Thermo Fisher Scientific
Miser™ Antibody Extender Solution NC is a post-transfer nitrocellulose membrane-treatment reagent that enables Western blot detection with much less primary antibody.
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Western Blotting Substrate, Pierce™ ECL Plus
Supplier: Thermo Fisher Scientific
The Pierce™ ECL Plus Western Blotting Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for Western blot detection using X-ray film or CCD- or laser-based imagers.
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Chemiluminescent RNA EMSA kit, LightShift™
Supplier: Thermo Fisher Scientific
LightShift™ Chemiluminescent RNA EMSA Kit provides a non-radioactive solution for studying RNA-protein interactions using an electrophoretic mobility-shift assay (EMSA).
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High molecular weight transfer buffer (5X)
Supplier: G-Biosciences
High molecular weight transfer buffer (5X) is designed to facilitate the transfer of notoriously difficult high molecular weight proteins (>70 kDa) during Western blotting.
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CN/DAB substrate kit, Pierce™
Supplier: Thermo Fisher Scientific
Pierce™ CN/DAB Substrate Kit is for chromogenic detection of horseradish peroxidase in immunohistochemistry and immunoblotting applications. The formed product is a black precipitate that photographs well and is useful in double staining applications. The coloured product is soluble in xylenes but not soluble in aqueous buffer such as Tris or phosphate. This combination of DAB and CN has a synergistic effect that results in greater sensitivity than either reagent alone.
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Substrate for western blotting, 1-Step™ NBT/BCIP
Supplier: Thermo Fisher Scientific
The 1-Step NBT/BCIP is a one-component sensitive precipitating alkaline phosphatase substrate for immunoblotting, immunohistochemistry, and in situ hybridisation applications.
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Substrate for western blotting, 1-Step™ NBT/BCIP plus suppressor
Supplier: Thermo Fisher Scientific
The 1-Step NBT/BCIP plus suppressor is a one-component alkaline phosphatase substrate that inhibits endogenous phosphatase activity. This substrate reacts with calf intestinal alkaline phosphatase to produce a dark purple precipitate and is ideal for immunohistochemistry and in situ hybridisation applications.
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NS/K Fluorogenic substrate kit, QuantaBlu™, Pierce™
Supplier: Thermo Fisher Scientific
QuantaBlu™ NS/K Fluorogenic Substrate Kit is a two-component kit containing proprietary fluorescent substrate and peroxide solutions for non-stopped kinetic assays.
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DNA ladder 250 to 10000 bp, AMPIGENE®
Supplier: Enzo Life Sciences
AMPIGENE® DNA ladder contains DNA fragments ranging from 250 to 10000 bp that can be used as molecular weight standards for agarose gel electrophoresis. The bands are evenly spaced and are intensified at 1000 bp and 3000 bp to serve as easily identifiable reference bands. The approximate mass of DNA in each of the bands is provided, assuming 5 µl load, for determining the approximate mass of DNA in samples with comparable intensity and sizes.
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DNA marker, pBR322 DNA/BsuRI (HaeIII) marker
Supplier: Thermo Fisher Scientific
pBR322 DNA/BsuRI (HaeIII) marker is suitable for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non denaturing polyacrylamide gels.
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1 kb DNA Ladder, Abcam
Supplier: Abcam
1 kb DNA Ladder ab286887 was previously known as M1157.
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DNA ladder, O'RangeRuler™
Supplier: Thermo Fisher Scientific
O'RangeRuler™ DNA ladders are step ladders that consist of basic unit repeats of 10, 15, 20, 50, 100, 200, or 500 bp. The ladders have a higher density of bands at a specific DNA size range and are therefore suitable for more accurate sizing of DNA in agarose or non denaturing polyacrylamide gels.
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Lambda DNA/HindIII Marker
Supplier: Thermo Fisher Scientific
Thermo Scientific Lambda DNA/HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.