You searched for: Electrophoresis Reagents

Supplier: G-Biosciences

Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.

Supplier: Abcam

NBT / BCIP chromogen (ab7468): a sensitive substrate for alkaline phosphatase detection in immunoblotting.

Supplier: VWR Chemicals

Protein Marker II for accurate molecular weight determination of proteins by SDS-PAGE.

Supplier: VWR Chemicals

RiboReady™ Color Micro RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels. The apparent sizes of bands in the RiboReady™ Color Micro RNA ladder are 100, 75, 50, 40, 30 and 20 bases in length.

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

Supplier: VWR Chemicals

Ladder with 13 fragments ranging from 100 to 3000 bp for easy band identification.

Supplier: VWR Chemicals

EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.

Supplier: VWR Chemicals

Protein Marker V (pre-stained) for monitoring protein separation, transfer efficiency and size determination.

Supplier: VWR Chemicals

EZ-Vision® 1 kb DNA ladder is a pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualisation dye that can be applied directly to the gel. This non toxic, non mutagenic dye eliminates the need for ethidium bromide staining. The 1 kb ladder consists of 10 double-stranded bands with molecular weights between 200 and 10 000 bp. The intensity of individual bands varies to facilitate easy identification.

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

Supplier: VWR Chemicals

DNA marker consists of 11 fragments ranging from 500 to 10000 bp for easy band identification.

Supplier: VWR Chemicals

peqGOLD pre-stained protein marker IV is a mixture of 10 recombinant, highly purified coloured proteins supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS-polyacrylamide gels.

Supplier: VWR Chemicals

peqGOLD DNA ladder 100 bp plus is ideal for size determination in the range of 100 to 3000 base pairs. There are no unspecific bands besides the fragments described. 1000 and 500 bp bands have a higher DNA content and serve as a size reference.

Supplier: VWR Chemicals

PCR DNA Marker™ offers 8 fragments ranging from 50 to 2000 bp. The marker contains high intensity reference bands that are ideal for quick sizing of PCR products and restriction digests. Simply mix 10 μl of PCR DNA Marker™ with required quantity of loading buffer and load into well. There is sufficient material for 100 assays.

Supplier: VWR Chemicals

VWR® DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

Supplier: VWR Chemicals

RiboReady™ Color 1 kb RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels.

Supplier: VWR Chemicals

VWR® DNA ladders are convenient dsDNA ladders supplied in a loading buffer, which are ready to use on agarose and SDS DNA gels.

Supplier: VWR Chemicals

peqGOLD pre-stained Protein Marker VI is a mixture of 10 coloured proteins. These proteins are recombinantly produced in E.coli, highly purified and supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS polyacrylamide gels.

Supplier: VWR Chemicals

peqGOLD Protein Marker I is a mixture of seven purified proteins which are re-dissolved 'ready to use' in loading buffer. The proteins resolve into sharp, tight bands in the range of 14,4 to 116,0 kDa when analysed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250.

Supplier: VWR Chemicals

Ladder with 10 fragments ranging from 50 to 500 bp for easy band identification. Ready to use formula pre-mixed with loading dye.

Supplier: Thermo Fisher Scientific

Although optimising assay conditions is the best way to achieve optimum results, re-performing the gel electrophoresis process to test each new primary antibody or antibody concentration is time-consuming and expensive. Restore™ Western blot stripping buffer offers an effective alternative.

Supplier: VWR Chemicals

Protein Marker III for monitoring protein separation, transfer efficiency and size determination.

Supplier: VWR Chemicals

High-Range DNA MW (molecular weight) marker offers 10 fragments ranging from 1503 to 48 502 bp. There is sufficient material for 75 to 150 assays.

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

Supplier: Thermo Fisher Scientific

SuperSignal™ West Femto maximum sensitivity substrate enables detection of low-femtogram amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies.

Supplier: Thermo Fisher Scientific

Some antibodies are difficult to remove from Western blots and require Longer incubation times or incubation temperatures greater than 22 °C. Restore™ PLUS Western Blot Stripping Buffer was developed to reduce incubation times while keeping incubations at room temperature. High-affinity antibodies can be quickly and effectively stripped from Western blots without removing transferred proteins, thereby allowing multiple reprobes of the target.

Supplier: Thermo Fisher Scientific

Guardian™ Peroxidase conjugate stabiliser/diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long term storage.

Supplier: Thermo Fisher Scientific

NBT (nitro-blue tetrazolium chloride) yields a blue-purple, water-insoluble product upon reduction of the tetrazolium salt. The product of reduction is NBT-formazan. This substrate is widely used for immunochemistry applications because the color is linear and stable. NBT is used as the substrate for both glucose oxidase and alkaline phosphatase systems.

Supplier: Thermo Fisher Scientific

The Fast Western Blot Kit, ECL Substrate contains optimised reagents that shorten the time which is required to perform a typical chemiluminescent Western blot from 4 hours down to approximately 55 minutes.